Pinoxaden resistance in Lolium perenne L. is due to both target-site and non-target-site mechanisms
Pinoxaden resistance in Lolium perenne L. is due to both target-site and non-target-site mechanisms
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DOI：
10.1016/j.pestbp.2022.105103
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发表时间：
2022-06-14
影响因子：
4.7
通讯作者：
Ngow, Zachary
中科院分区：
文献类型：
--
作者：
Ghanizadeh, Hossein;Buddenhagen, Christopher E.;Ngow, Zachary
施用抑制乙酰辅酶A羧化酶（ACCase）的除草剂是选择性防除多年生黑麦草（Loliumperenne L.）在新西兰的小麦和大麦作物中。在这项研究中，我们已经证实和特点的抗药性pinoxaline，ACCase抑制除草剂，在人口的L。多年生植物进行了剂量反应实验，以评估皮诺沙丹的抗性水平，并根据LD 50值，研究的人口是41.4倍，比敏感人口皮诺沙丹的抗性。应用马拉硫磷，细胞色素P450的抑制剂，前pinoxetrin治疗的抗性水平降低到9.7倍。然而，预处理谷胱甘肽S-转移酶（GST）抑制剂4-氯7-硝基苯并恶二唑之前，皮诺治疗并不影响皮诺耐药性。ACCase基因的部分测序显示，抗性群体在位置2041处具有异亮氨酸至缬氨酸的置换。这些结果表明，细胞色素P450为基础的和靶位点的机制是共同与这种情况下，在L。多年生植物抗唑啉菌的L.多年生禾草对精喹禾灵也有抗性（108.6倍），但对烯草酮敏感，因此可以用烯草酮来治理这种唑啉草丹抗性的禾草。多年生植物这是首次报道L.多年生植物种群，其中一个罕见的靶位点突变与增强的细胞色素P-450活性协同作用，从而赋予皮诺沙星抗性。对ACCase抑制型除草剂抗性的进化。多年生植物种群表明，需要综合杂草管理措施，以防止在新西兰谷类作物系统中广泛的抗性发展。
Application of herbicides inhibiting acetyl CoA carboxylase (ACCase) has been one of the main strategies for selectively controlling grass weed species such as perennial ryegrass (Lolium perenne L.) in wheat and barley crops in New Zealand. In this study, we have confirmed and characterized resistance to pinoxaden, an ACCase-inhibiting herbicide, in a population of L. perenne. Dose-response experiments were conducted to assess the level of pinoxaden resistance, and based on the LD50 values, the studied population was 41.4-times more resistant to pinoxaden than a susceptible population. Application of malathion, an inhibitor of the cytochrome P450s, preceding pinoxaden treatment reduced the level of resistance to 9.7-fold. However, pre-treatment with the glutathione S-transferase (GST) inhibitor 4-chloro7- nitrobenzoxadiazole prior to pinoxaden treatment did not affect pinoxaden resistance. Partial sequencing of the ACCase gene revealed that the resistant population had an isoleucine to valine replacement at position 2041. These results suggest that both cytochrome P450-based and target-site mechanisms are jointly associated with this instance of pinoxaden resistance in L. perenne. The pinoxaden-resistant L. perenne individuals were also resistant to quizalofop-p-ethyl (108.6-fold), but they were susceptible to clethodim, which can, therefore, be used to manage this pinoxaden-resistant L. perenne. This is the first report of a L. perenne population in which a rare target-site mutation works in concert with enhanced cytochrome P-450 activity to confer pinoxaden resistance. Evolution of resistance to ACCase-inhibiting herbicides in this L. perenne population indicates that integrated weed management practices are required to prevent widespread resistance developing in New Zealand cereal crop systems.