Optical waveguide lightmode spectroscopy immunosensor for detection of carp vitellogenin
Optical waveguide lightmode spectroscopy immunosensor for detection of carp vitellogenin
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DOI：
10.1016/j.snb.2012.10.079
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发表时间：
2013-01-01
影响因子：
8.4
通讯作者：
Szekacs, Andras
中科院分区：
文献类型：
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作者：
Adanyi, Nora;Majer-Baranyi, Krisztina;Szekacs, Andras
建立了无标记光波导光模式光谱(OWLS)免疫传感器，用于鲤鱼体内卵黄蛋白原(VTG)的检测。将从鲤鱼卵巢中提纯的抗卵黄蛋白(LPV)多克隆血清直接和间接测定，并对其分析特性(检出限、特异度、基质效应)进行了研究。建立的OWLS直接免疫传感器和夹心ELISA法分别在600-12,000 ng-1和200-3000 ng-1标准溶液中LPV浓度范围内呈良好的线性关系。竞争免疫传感器对LPV的灵敏检测范围为3到100 ng mL(-1)。用间接猫头鹰方法测定了雌、雄鲤鱼血液和肝脏中VTG的含量。为了减少基质效应，使用添加了LPV的雄性鲤鱼的不含VTG的血液和肝脏样本，得到了校准曲线。雌性鲤鱼肝脏中的VTG水平低于血液样本中的VTG水平，因此，由于可能暴露于水污染的内分泌干扰化学物质，血液是监测雄性鲤鱼VTG升高的更合适的组织。(C)2012爱思唯尔B.V.保留所有权利。
Label-free, optical waveguide lightmode spectroscopy (OWLS) based immunosensors were developed for vitellogenin (Vtg) determination in carp (Cyprinus carpio). Purified polyclonal serum raised in rabbits against lipovitellin (Lpv) purified from carp ovary and shown to fully cross-react with Vtg was applied both in direct and indirect measurement formats, and analytical features (limit of detection, specificity, matrix effects) were investigated. The direct OWLS immunosensor and the sandwich ELISA assay developed showed linear measuring range in 600-12,000 ng mL(-1) and 200-3000 ng mL(-1) Lpv concentrations in standard solutions, respectively. The competitive immunosensor allowed a sensitive detection range for Lpv between 3 and 100 ng mL(-1). Vtg content in blood and liver samples from male and female carp were determined by indirect OWLS. To reduce the matrix effect, calibration curves were obtained using Vtg-free blood and liver samples from male carp spiked with Lpv. Vtg levels in the liver of female carp was lower than determined in the blood samples, therefore, blood occurs to be a more proper tissue to monitor elevated Vtg in male carp due to possible exposure to water-contaminating endocrine disrupting chemicals. (C) 2012 Elsevier B. V. All rights reserved.