Colonization and community structure of root-associated microorganisms of Sabina vulgaris with soil depth in a semiarid desert ecosystem with shallow groundwater
Colonization and community structure of root-associated microorganisms of Sabina vulgaris with soil depth in a semiarid desert ecosystem with shallow groundwater
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DOI：
10.1007/s00572-011-0417-8
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发表时间：
2011-11
期刊：
影响因子：
3.9
通讯作者：
T. Taniguchi;H. Usuki;J. Kikuchi;M. Hirobe;N. Miki;K. Fukuda;Guosheng Zhang;Linhe Wang;K. Yoshikawa;N. Yamanaka
中科院分区：
文献类型：
--
作者：
T. Taniguchi;H. Usuki;J. Kikuchi;M. Hirobe;N. Miki;K. Fukuda;Guosheng Zhang;Linhe Wang;K. Yoshikawa;N. Yamanaka
在干旱地区的深层土壤中观察到丛枝菌根真菌（AMF）。然而，AMF 群落结构随土壤深度的变化以及其他根相关微生物的垂直分布尚不清楚。在这里，我们研究了 AMF 和深色隔膜真菌 (DSF) 的定殖，以及 AMF 和内生真菌 (EF) 和内生细菌 (EB) 的群落结构与地下水浅的半干旱沙漠土壤深度的关系。以 20 厘米的间隔从地表到地下水位收集柏树的根和土壤。测量了土壤化学成分（含水量、总氮和速效磷）以及 AMF 和 DSF 的定植情况。通过末端限制性片段长度多态性分析检查AMF、EF和EB的群落结构。 AMF定植率随着土壤深度的增加而减少，但大多高于50%。 AMF 系统发育型的数量随着土壤深度的增加而减少，但在深度达 100 cm 时观察到超过 5 种系统发育型。 AMF系统型数量与土壤含水量在0%~15%范围内呈显着正相关。 DSF 定植率很高，但仅限于土壤表面。即使在深层土壤中，EF和EB的系统发育型数量也存在差异，其群落组成与AMF的定殖和群落组成相关。本研究表明，在半干旱地区，根部AMF物种丰富度下降，但在深层土壤中保持不变，并且AMF定植和群落结构的变化与其他根部相关微生物的群落结构相关。
Arbuscular mycorrhizal fungi (AMF) have been observed in deep soil layers in arid lands. However, change in AMF community structure with soil depth and vertical distributions of the other root-associated microorganisms are unclear. Here, we examined colonization by AMF and dark septate fungi (DSF), as well as the community structure of AMF and endophytic fungi (EF) and endophytic bacteria (EB) in association with soil depth in a semiarid desert with shallow groundwater. Roots ofSabina vulgarisand soils were collected from surface to groundwater level at 20-cm intervals. Soil chemistry (water content, total N, and available P) and colonization of AMF and DSF were measured. Community structures of AMF, EF, and EB were examined by terminal restriction fragment length polymorphism analysis. AMF colonization decreased with soil depth, although it was mostly higher than 50%. Number of AMF phylotypes decreased with soil depth, but more than five phylotypes were observed at depths up to 100 cm. Number of AMF phylotypes had a significant and positive relationship with soil moisture level within 0–15% of soil water content. DSF colonization was high but limited to soil surface. Number of phylotypes of EF and EB were diverse even in deep soil layers, and the community composition was associated with the colonization and community composition of AMF. This study indicates that AMF species richness in roots decreases but is maintained in deep soil layers in semiarid regions, and change in AMF colonization and community structure associates with community structure of the other root-associated microorganisms.