Evaluation of the efficacy of a novel Vibrio vulnificus vaccine based on antibacterial peptide inactivation in turbot, Scophthalmus maximus.
Evaluation of the efficacy of a novel Vibrio vulnificus vaccine based on antibacterial peptide inactivation in turbot, Scophthalmus maximus.
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DOI：
10.1016/j.fsi.2021.09.008
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发表时间：
2021-09
影响因子：
4.7
通讯作者：
Qin-Qin Gu-Qin;Guang-hua Wang;Ningqiu Li;Dongfang Hao;Hongmei Liu;Chang-biao Wang;Yong-hua Hu;M. Zhang
中科院分区：
文献类型：
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作者：
Qin-Qin Gu-Qin;Guang-hua Wang;Ningqiu Li;Dongfang Hao;Hongmei Liu;Chang-biao Wang;Yong-hua Hu;M. Zhang
Tongue sole tissue factor pathway inhibitor 2（TFPI-2）C-末端衍生肽TC 38先前已被证明可杀灭创伤弧菌细胞而不裂解细胞膜;因此，剩余的细菌外壳具有作为灭活疫苗的潜在应用。因此，本研究旨在评估由新病毒诱导的免疫应答。创伤疫苗TC38-killedV.在大菱鲆模型中检测创伤细胞（TKC）。鱼肌肉注射TKC或FKC（福尔马林灭活V. vulnificuscells）并用致死剂量的V.创伤。结果表明，与FKC相比，TKC具有较好的保护效果。创伤感染，相对存活率分别为53.29%和63.64%。免疫学分析显示，与FKC组和对照组相比，TKC组在免疫后7 d表现出：（1）巨噬细胞呼吸爆发能力和杀菌活性显著增强，（2）碱性磷酸酶、酸性磷酸酶、溶菌酶和总超氧化物歧化酶水平显著升高，（3）巨噬细胞吞噬功能显著增强。（3）血清凝集抗体滴度越高，相应的血清杀菌能力越强，血清凝集作用越强，IgM表达水平也越高;（4）免疫相关基因的高表达，这些基因参与了先天免疫和获得性免疫。综上所述，这是第一个研究开发一个novelV。TKC是一种有效的创伤灭活疫苗。vulnificusinfection为水产养殖。
Tongue sole tissue factor pathway inhibitor 2 (TFPI-2) C-terminus derived peptide, TC38, has previously been shown to killVibriovulnificuscells without lysing the cell membrane; thus, the remaining bacterial shell has potential application as an inactivated vaccine. Therefore, this study aimed to evaluate the immune response induced by the novelV. vulnificusvaccine. The protective potential of TC38-killedV. vulnificuscells (TKC) was examined in a turbot model. Fish were intramuscularly vaccinated with TKC or FKC (formalin-killedV. vulnificuscells) and challenged with a lethal-dose ofV. vulnificus. The results showed that compared with FKC, TKC was effective in protecting fish againstV. vulnificusinfection, with relative percent of survival (RPS) rates of 53.29% and 63.64%, respectively. The immunological analysis revealed that compared with the FKC and control groups, the TKC group exhibited: 1) significantly higher respiratory burst ability and bactericidal activity of macrophages at 7 d post-vaccination; 2) increased alkaline phosphatase, acid phosphatase, lysozyme, and total superoxide dismutase levels post-vaccination; 3) higher serum agglutinating antibody titer with corresponding higher serum bactericidal ability, and a more potent serum agglutination effect, as well as an increased IgM expression level; 4) higher expression of immune relevant genes, which were involved in both innate and adaptive immunity. Taken together, this is the first study to develop a novelV. vulnificusinactivated vaccine based on AMP inactivation, and TKC is an effective vaccine againstV. vulnificusinfection for aquaculture.