Polymorphism of trichothecene biosynthesis genes in deoxynivalenol- and nivalenol-producing Fusarium graminearum isolates.
Polymorphism of trichothecene biosynthesis genes in deoxynivalenol- and nivalenol-producing Fusarium graminearum isolates.
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DOI：
10.1017/s0953756203007317
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发表时间：
2003-02
影响因子：
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通讯作者：
Hye-Seon Kim;T. Lee;M. Dawlatana;S. Yun;Yin-Won Lee
中科院分区：
文献类型：
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作者：
Hye-Seon Kim;T. Lee;M. Dawlatana;S. Yun;Yin-Won Lee
用化学和分子生物学方法研究了167个禾谷镰刀菌（Fusarium graminearum）菌株产青霉烯毒素的多样性。分离大麦，玉米，小麦生长在韩国生产脱氧雪腐镰刀菌烯醇（DON）或雪腐镰刀菌烯醇（NIV），而分离玉米生长在美国生产的DON。使用Tri 5的0.6kb片段作为探针，对来自这些分离物的MseI消化的基因组DNA进行Southern印迹，Tri 5是一种用于产生二萜类化合物的关键酶。该技术揭示了这些菌株之间的单带多态性，1.8-和2.2-kb的带所产生的DON和NIV生产商，分别。使用Tri 7或Tri 13衍生的引物对同一组分离株进行先前开发的PCR检测。这些试验还揭示了单带多态性之间的NIV和DON生产化学型。在Tri 5，Tri 7，或Tri 13在所有的美国分离株的多态性是一致的，其化学型通过GC-MS鉴定。然而，7个韩国分离株，化学和分子分析产生了看似不一致的结果。这个问题通过使用Tri 5探针的Southern印迹分析和跨越Tri 5的3.8kb区域的序列比较来解决。此外，这些特殊的分离株之一被发现携带DON和NIV化学型特异性区域，可能是由于两种化学型之间的重组。
Diversity in trichothecene mycotoxin production by 167 isolates of Fusarium graminearum was examined by chemical and molecular methods. Isolates from barley, corn, and wheat grown in Korea produced either deoxynivalenol (DON) or nivalenol (NIV), whereas isolates from corn grown in the United States produced DON only. Southern blotting of MseI-digested genomic DNA's from these isolates was performed using a 0.6-kb fragment of Tri5, a key enzyme for trichothecene production, as a probe. This technique revealed a single-band polymorphism between these isolates, with 1.8- and 2.2-kb bands arising from DON and NIV producers, respectively. The same set of isolates was subjected to previously developed PCR assays using primers derived from Tri7 or Tri13. These assays also revealed a single-band polymorphism between NIV- and DON-producing chemotypes. The polymorphisms at Tri5, Tri7, or Tri13 in all of the US isolates were consistent with their chemotypes as identified by GC-MS. However, for seven Korean isolates, chemical and molecular analyses yielded seemingly inconsistent results. This issue was resolved by Southern blot analysis with the Tri5 probe using two other restriction enzymes and sequence comparison of a 3.8-kb region spanning Tri5. In addition, one of these exceptional isolates was found to carry both DON and NIV chemotype-specific regions, possibly resulting from recombination between the two chemotypes.