Quantitative Bioluminescence Tomography for In Vivo Volumetric-Guided Radiotherapy.
Quantitative Bioluminescence Tomography for In Vivo Volumetric-Guided Radiotherapy.
复制标题
DOI：
10.1007/978-1-0716-1803-5_38
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
包括我们在内的几个小组已经开始努力开发用于人类治疗的模拟放射治疗（RT）的小动物照射器。用于引导照射的主要图像模式是锥束计算机断层扫描（CBCT）。虽然CBCT提供了出色的制导能力，但在低图像对比度环境下，它不太擅长定位软组织目标。相比之下，生物发光成像（BLI）提供了强大的图像对比度，因此是软组织靶向的一个有吸引力的解决方案。然而，通常使用的动物表面二维BLI不足以指导照射，因为生物发光肿瘤内部的光传输极易受到光程长度和组织吸收和散射的影响。认识到这些局限性，我们将3D生物发光断层扫描（BLT）与小动物辐射研究平台（SARRP）结合起来。在本章中，我们介绍了定量BLT (QBLT)，它具有量化肿瘤体积的先进能力，可以用于照射指导。详细介绍了系统组件、校准方案和逐步进行qblt引导辐照的程序。
Several groups, including ours, have initiated efforts to develop small-animal irradiators that mimic radiation therapy (RT) for human treatment. The major image modality used to guide irradiation is cone-beam computed tomography (CBCT). While CBCT provides excellent guidance capability, it is less adept at localizing soft tissue targets growing in a low image contrast environment. In contrast, bioluminescence imaging (BLI) provides strong image contrast and thus is an attractive solution for soft tissue targeting. However, commonly used 2D BLI on an animal surface is inadequate to guide irradiation, because optical transport from an internal bioluminescent tumor is highly susceptible to the effects of optical path length and tissue absorption and scattering. Recognition of these limitations led us to integrate 3D bioluminescence tomography (BLT) with the small animal radiation research platform (SARRP). In this chapter, we introduce quantitative BLT (QBLT) with the advanced capabilities of quantifying tumor volume for irradiation guidance. The detail of system components, calibration protocol, and step-by-step procedure to conduct the QBLT-guided irradiation are described.