Real-time in vivo imaging of platelets, tissue factor and fibrin during arterial thrombus formation in the mouse
Real-time in vivo imaging of platelets, tissue factor and fibrin during arterial thrombus formation in the mouse
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DOI：
10.1038/nm782
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发表时间：
2002-10-01
期刊：
影响因子：
82.9
通讯作者：
Furie, B
中科院分区：
文献类型：
--
作者：
Falati, S;Gross, P;Furie, B
我们使用共聚焦显微镜和宽视场显微镜对活体小鼠微循环中血栓的形成进行了实时成像。该系统提供高速、几乎同时采集多个荧光探头和一个亮场通道的图像。血管损伤是通过显微镜光学聚焦的激光引起的。我们在单个发展中的血栓中观察了激光诱导内皮损伤后的血小板沉积、组织因子积聚和纤维蛋白生成。体内血液凝固的启动需要组织因子在发展中的血栓的上游和血栓-血管壁界面上最初积聚。随后，组织因子与血栓内部相关。组织因子具有生物活性，与血栓内纤维蛋白的生成有关。
We have used confocal and widefield microscopy to image thrombus formation in real time in the microcirculation of a living mouse. This system provides high-speed, near-simultaneous acquisition of images of multiple fluorescent probes and of a brightfield channel. Vascular injury is induced with a laser focused through the microscope optics. We observed platelet deposition, tissue factor accumulation and fibrin generation after laser-induced endothelial injury in a single developing thrombus. The initiation of blood coagulation in vivo entailed the initial accumulation of tissue factor on the upstream and thrombus-vessel wall interface of the developing thrombus. Subsequently tissue factor was associated with the interior of the thrombus. Tissue factor was biologically active, and was associated with fibrin generation within the thrombus.