Comparative analysis of the chloroplast proteomes of a wheat (Triticum aestivum L.) single seed descent line and its parents
Comparative analysis of the chloroplast proteomes of a wheat (Triticum aestivum L.) single seed descent line and its parents
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小麦（Triticum aestivum L.）单籽世系及其亲本叶绿体蛋白质组的比较分析
DOI：
10.1016/j.jplph.2013.03.016
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发表时间：
2013-09-01
影响因子：
4.3
通讯作者：
Mi, Hualing
中科院分区：
文献类型：
--
作者：
He, Zhi-Hui;Li, Hong-Wei;Mi, Hualing
利用2-DE、MALDI-TOF MS和MS/MS技术比较了小麦单粒下降系10(SSDL10)及其亲本的叶绿体蛋白质组和叶绿体蛋白质组，在2D凝胶中可重复检测到300多个蛋白质点。SSDL10与亲本的差异表达有18个点，其中16个点被MS鉴定为在叶绿体中定位。这些蛋白质分为不同的功能类别，包括光合作用中的Calvin循环和电子传递、氧化还原动态平衡、代谢和调节。除Rubisco大亚基外，SSDL10的叶绿素a-b结合蛋白、ATP合成酶德尔塔亚基、铁氧还蛋白-NADP(+)氧化还原酶(FNR)等光合作用电子传递物质的含量均高于其亲本。此外，SSDL10中围绕光系统I(CET)的循环电子传递比亲本更快。NADPH-NBT氧化还原酶活性分析结合免疫检测进一步表明，SSDL10的CET高于亲本，可能与含有FNR的两个高分子量蛋白质复合体的活性有关。讨论了CET调控SSDL10光合作用的可能机制。(C)2013年爱思唯尔股份有限公司。版权所有。
To understand the photosynthetic basis in a single seed descent line 10 (SSDL10) of wheat contained high ATP in leaves, the chloroplast proteome was compared to SSDL10 and its parents using a combination of 2-DE and MALDI-TOF MS and MS/MS. More than 300 protein spots could be reproducibly detected in the 2D gel. 18 spots were differentially expressed between SSDL10 and the parents, 16 of which were identified by MS with the localization in chloroplasts. These proteins are grouped into diverse functional categories, including Calvin cycle and electron transport in photosynthesis, redox homeostasis, metabolism, and regulation. In addition to Rubisco large subunit, the content of photosynthetic electron transfers such as chlorophyll a-b binding protein, ATP synthase delta subunit, ferredoxin-NADP(+) oxidoreductase (FNR) was higher in SSDL10 than in its parents. Furthermore, cyclic electron transfer around photosystem I (CET) was faster in SSDL10 than in the parents. Analysis of NADPH-NBT oxidoreductase activity combined with immuno-detection further revealed that, the activity of two high molecular mass protein complexes containing FNR probably involved, the CET appeared higher in SSDL10 than in the parents. The possible mechanism for the regulative role of CET in photosynthesis in SSDL10 is discussed. (C) 2013 Elsevier GmbH. All rights reserved.