Microfluidic system for dielectrophoretic separation based on a trapezoidal electrode array
Microfluidic system for dielectrophoretic separation based on a trapezoidal electrode array
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DOI：
10.1039/b505088j
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发表时间：
2005-01-01
期刊：
影响因子：
6.1
通讯作者：
Park, JK
中科院分区：
文献类型：
--
作者：
Choi, S;Park, JK
提出了一种基于梯形电极阵列（TEA）的新型微流控介电泳分离装置。在该方法中，具有不同介电特性的颗粒通过由用于负介电泳（DEP）下的颗粒的介电泳偏转的TEA和具有弯曲和扩展区域的聚二甲基硅氧烷（PDMS）微流体通道组成的装置分离。聚苯乙烯微粒暴露于微流体通道中的TEA产生的电场，并被分散聚焦以使它们全部排列到一个侧壁。当这些颗粒到达另一个TEA的区域进行介电泳分离时，由于它们不同的介电泳速度，它们沿着与流体流垂直的方向以不同的位置沿着分离。为了评价分离过程和性能，研究了流速对介电电泳聚焦的影响和梯形电极数量对介电电泳分离的影响。由于该方法利用TEA作为负介电电泳的来源，因此可以防止非特异性颗粒粘附在电极表面;依赖于正介电电泳力的传统分离方法都会遇到这个问题。此外，由于连续分离各种颗粒类型，因此该方法可以容易地应用于各种电介质颗粒的分离和分析，具有高颗粒回收率和选择性。
This paper presents a novel microfluidic device for dielectrophoretic separation based on a trapezoidal electrode array (TEA). In this method, particles with different dielectric properties are separated by the device composed of the TEA for the dielectrophoretic deflection of particles under negative dielectrophoresis (DEP) and poly(dimethylsiloxane) (PDMS) microfluidic channel with a sinuous and expanded region. Polystyrene microparticles are exposed to an electric field generated from the TEA in the microfluidic channel and are dielectrophoretically focused to make all of them line up to one sidewall. When these particles arrive at the region of another TEA for dielectrophoretic separation, they are separated having different positions along the perpendicular direction to the fluid flow due to their different dielectrophoretic velocities. To evaluate the separation process and performance, both the effect of the flow rate on dielectrophoretic focusing and the influence of the number of trapezoidal electrodes on dielectrophoretic separation are investigated. Now that this method utilizes the TEA as a source of negative DEP, non-specific particle adhering to the electrode surface can be prevented; conventional separation approaches depending on the positive DEP force suffer from this problem. In addition, since various particle types are continuously separated, this method can be easily applicable to the separation and analysis of various dielectric particles with high particle recovery and selectivity.