Peripheral Immune Response in Pulmonary Tuberculosis
Peripheral Immune Response in Pulmonary Tuberculosis
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DOI：
10.1111/j.1365-3083.2009.02294.x
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发表时间：
2009-09-01
影响因子：
3.7
通讯作者：
Deniz, G.
中科院分区：
文献类型：
--
作者：
Aktas, E.;Ciftci, F.;Deniz, G.
细胞免疫反应和迟发型超敏反应在肺结核的免疫发病机制中起重要作用。但确切的机制仍有待澄清。Th 1细胞主要参与PTB的细胞免疫应答，并提供正常的愈合过程，具有最小或无后遗症，而Th 2细胞和CD 8（+）T淋巴细胞应答可能导致更严重的疾病类型。在这项研究中，我们研究了外周血免疫反应的PTB。研究组由抗酸阳性的PTB和HIV血清学阴性的年轻男性士兵组成。对照组为无PTB病史的健康男性志愿者。流式细胞术测定CD 8（+）T细胞和淋巴细胞胞浆内细胞因子IL-2、IL-4、IL-5、IL-10和IFN-γ含量，流式细胞术微球阵列（CBA）测定血清IL-2、IL-4、IL-5、IL-10、IFN-γ和TNF-α水平。两组患者T、B、NK细胞百分率及HLA-DR表达差异无统计学意义，但PT B组CD 3（+）HLA-DR+活化T细胞百分率高于健康对照组。与健康对照组相比，PTB患者淋巴细胞和IFN-γ（+）CD 8（+）T细胞中IL-2、IL-4、IL-5、IL-10含量显著降低，同时，PTB患者血清IL-2、IL-4、IL-5和TNF-α水平也显著降低。结论：产生Th 1和Th 2型细胞因子的CD 8（+）T细胞可能在结核分枝杆菌的外周免疫应答中起重要作用。
Cellular immune response and delayed-type hypersensitivity reactions are considered to play a major role in the immunopathogenesis of pulmonary tuberculosis (PTB). But the exact mechanism is still to be clarified. Th1 cells are mainly involved in cellular immune responses in PTB and provide a normal healing process with minimal or no sequela whereas Th2 cell and CD8(+) T lymphocyte responses may lead to more severe type of disease. In this study, we investigated the peripheral blood immune responses in PTB. The study group consisted of acid fast positive young male soldiers with PTB and a negative HIV serology. The control group included healthy young volunteer male soldiers without a history of PTB. Intracytoplasmic cytokine content of CD8(+) T cells and lymphocytes, including IL-2, IL-4, IL-5, IL-10 and IFN-gamma were determined by flow cytometry, and IL-2, IL-4, IL-5, IL-10, IFN-gamma and TNF-alpha serum levels were measured by cytometric bead array (CBA). No difference was observed between the percentages of T, B, NK cells and HLA-DR expression in both groups, however, the number of CD3(+)HLA-DR+ activated T cell percentages was higher in PTB group as compared to healthy subjects. IL-2, IL-4, IL-5, IL-10 contents of lymphocytes and IFN-gamma(+)CD8(+) T cells were found to be significantly lower in PTB patients when compared with healthy subjects, and in parallel, serum IL-2, IL-4, IL-5 and TNF-alpha levels were also significantly lower in PTB patients. In conclusion we suggest that, CD8(+) T cells producing both Th1 and Th2 type cytokines, may play important role in the peripheral immune response to mycobacteria.