High ambient temperature leads to reduced FT expression and delayed flowering in Brassica rapa via a mechanism associated with H2A.Z dynamics
High ambient temperature leads to reduced FT expression and delayed flowering in Brassica rapa via a mechanism associated with H2A.Z dynamics
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DOI：
10.1111/tpj.14446
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发表时间：
2019-08-09
期刊：
影响因子：
7.2
通讯作者：
Crevillen, Pedro
中科院分区：
文献类型：
--
作者：
del Olmo, Ivan;Poza-Viejo, Laura;Crevillen, Pedro
开花时间是一个相关的农艺性状，因为它对种子和果实的最佳形成至关重要。控制这种发育阶段转换的遗传途径已在拟南芥中被广泛研究。这些途径集中在少数基因中，包括FT，即所谓的成花素，它整合了环境温度等环境线索。然而，关于芸苔属作物开花时间的详细和功能性研究很少。在这里，我们研究的FT芜菁同源物的作用和高环境温度对开花时间在这种作物的影响。表型特征和基因表达分析表明，BraA.FT.a（BraA02g016700.3C）是决定性的启动花的过渡，因此，braA.FT.a的功能丧失和亚型突变的结果在晚开花表型。我们还表明，高环境温度延迟B。通过降低BraA.FT.a表达来实现芜菁花的转变。引人注目的是，这些表达变化与组蛋白H2A.Z水平增加和BraA.FT.a基因座在高环境温度下不易接近的染色质构型相关。有趣的是，在高环境温度下也观察到其他B的H2A. Z水平增加。rapa温度响应基因。先前的报道界定了拟南芥在高环境温度下由于FT基因座中的H2A.Z掺入减少而提早开花。我们的数据揭示了B中保守的染色质介导机制。其中，在FT染色质处掺入H2A.Z以响应温暖的环境温度导致不同的开花时间响应。这项工作将有助于开发具有开花时间要求的改良芸苔属作物品种，以科普全球变暖。开放研究徽章这篇文章获得了开放材料徽章，因为它公开了再现所报告的程序和分析所需的研究方法的组成部分。有关方法，请访问。
Flowering time is a relevant agronomic trait because is crucial for the optimal formation of seeds and fruits. The genetic pathways controlling this developmental phase transition have been studied extensively in Arabidopsis thaliana. These pathways converge in a small number of genes including FT, the so-called florigen, which integrates environmental cues like ambient temperature. Nevertheless, detailed and functional studies about flowering time in Brassica crops are scarce. Here we study the role of the FT Brassica rapa homologues and the effect of high ambient temperature on flowering time in this crop. Phenotypic characterization and gene-expression analyses suggest that BraA.FT.a (BraA02g016700.3C) is decisive for initiating floral transition; consequently, braA.ft.a loss-of-function and hypomorphic mutations result in late flowering phenotypes. We also show that high ambient temperature delays B. rapa floral transition by reducing BraA.FT.a expression. Strikingly, these expression changes are associated with increased histone H2A.Z levels and less accessible chromatin configuration of the BraA.FT.a locus at high ambient temperature. Interestingly, increased H2A.Z levels at high ambient temperature were also observed for other B. rapa temperature-responsive genes. Previous reports delimited that Arabidopsis flowers earlier at high ambient temperature due to reduced H2A.Z incorporation in the FT locus. Our data reveal a conserved chromatin-mediated mechanism in B. rapa and Arabidopsis in which the incorporation of H2A.Z at FT chromatin in response to warm ambient temperature results in different flowering time responses. This work will help to develop improved Brassica crop varieties with flowering time requirements to cope with global warming. Open Research Badges This article has earned an Open Materials Badge for making publicly available the components of the research methodology needed to reproduce the reported procedure and analysis. Methods are available at .