Isolation, Identification and Prevalence of and S. constellatus from the Human Mouth
Isolation, Identification and Prevalence of and S. constellatus from the Human Mouth
复制标题
人类口腔中星座链球菌的分离、鉴定和流行情况
DOI：
10.3402/mehd.v6i6.8147
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发表时间：
1993
影响因子：
--
通讯作者：
G. Tillotsen
中科院分区：
文献类型：
--
作者：
R. Whiley;L. Freemantle;D. Beighton;J. Radford;J. Hardie;G. Tillotsen
本研究的目的是确定潜在致病性的咽峡炎链球菌、咽峡炎链球菌和链球菌的患病率和比例分布。intermedius和S.在人类口腔内的位置。来自162名不同受试者的样本包括龈上菌斑和唾液（18名受试者）、龈下样本（14名受试者）和漱口液（130名受试者）。在萘啶酸磺胺二甲嘧啶选择性琼脂上回收的所有三种菌种的回收率与在非选择性培养基（哥伦比亚血琼脂）上获得的回收率相当。S.咽峡炎菌是三种菌中分离频率最高的一种，占6.10%; intermedius和S.星座分别从53%和13%的受试者中恢复。S.在检查的所有口腔部位，咽峡炎菌的检出频率和数量均高于其他两个种属。S.除龈下样本中出现频率和数量与S相似外，Constellatus最不常见且数量较少。中间体大多数地点都有S. anginosus和S. intermedius单独或一起，而组合S。intermedius与S. constellatus或所有三个物种在一起，很少发生。最常见的地点只有一个物种的单一表型（78%的S。anginosus、S. intermedius和91%的S. constellatus生产网站）。一个物种的两个表型一起被发现的频率要低得多，三个表型很少从一个单一的网站检测。129株S.在本研究期间发现的10例心绞痛中，只有3例（2%）属于所谓的广泛发酵型（发酵甘露醇和棉子糖），以前与泌尿生殖源有关。关键词-咽峡炎链球菌，S.中间S.星座，口腔
This study was carried out to determine the prevalence and proportional distribution of the potentially pathogenic species Streptococcus anginosus, S. intermedius and S. constellatus at sites within the human mouth. The samples from 162 different subjects included supragingival plaque and saliva (18 subjects), subgingival samples (14 subjects) and mouthrinsings (130 subjects). All three species were recovered on nalidixic acid sulphamethazine selective agar at rates comparable with those obtained on a non-selective medium (Columbia blood agar). S. anginosus was the most frequently isolated of the three species, being found in 6.10 per cent of subjects examined, while S. intermedius and S. constellatus were recovered from 53 per cent and 13 per cent of subjects respectively. S. anginosus was recovered more frequently and in higher numbers at all oral sites examined than the other two species. S. constellatus was isolated least often and in lower numbers, except in the subgingival samples where it occurred as frequently and in similar numbers to S. intermedius. The majority of sites harboured S. anginosus and S. intermedius either singly or together, whilst the combination S. intermedius with S. constellatus or all three species together, occurred rarely. Sites most frequently harboured only a single phenotype of a species (78 percent of S. anginosus , 83 per cent of S. intermedius and 91 percent of S. constellatus yielding sites). Two phenotypes of a species were found together much less frequently and three phenotypes were rarely detected from a single site. Of the 129 isolates of S. anginosus recovered during this study, only three (2 per cent) were of the so-called broadly fermentative biotype (which ferment mannitol and raffinose), previously associated with urogenital sources. Keywords - Streptococcus anginosus, S. intermedius, S. constellatus , Oral cavity