An improved method of parabiosis
An improved method of parabiosis
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DOI：
10.1002/ar.1090570404
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发表时间：
1933-11-01
影响因子：
--
通讯作者：
Meyer, RK
中科院分区：
文献类型：
--
作者：
Bunster, E;Meyer, RK
联体共生的方法已被广泛用于研究许多物质的肱骨传输，并已在控制生殖道和性腺的激素的研究特别有用。通常使用的联体共生方法是Sauerbruck和Heyde（'08）描述的方法。这种方法包括将两只动物的皮肤和体腔连接起来（腹腔吻合术）。我们用Sauerbruck和Heyde的方法进行了一些初步实验，旨在研究雌激素对垂体前叶性腺刺激复合物的影响。所有手术均采用无菌技术进行，并对动物进行合理的术后护理。然而，我们的动物大量死亡或伤口严重感染，在动物生活的情况下，伴侣之间的皮肤拉伸并扭曲。(This在施密特的论文（'22）中说明了Sauerbruck和Heyde方法的特殊缺点。我们用Sauerbruck和Heyde所描述的方法得到了不令人满意的结果，对这些结果的研究使我们相信，在大多数情况下，感染和死亡是由动物拉扯皮肤缝合线引起的。因此，我们得出结论，动物之间的结合必须比皮肤和肌肉结合更牢固。因此
The method of parabiosis has been used extensively in the study of the humeral transmission of many substances and has been particularly useful in the study of the hormones controlling the reproductive tract and gonads. The method of parabiosis which has usually been used is that described by Sauerbruck and Heyde ('08). This method consists in uniting the skin and body cavities of the two animals (coelioanastomosis). We used the method of Sauerbruck and Heyde in a number of preliminary experiments which were designed to study the influence of oestrin on the gonad-stimulating complex of the anterior pituitary. The operations were all done with aseptic technique and reasonable post-operative care was taken of the animals. However, a large number of our animals died or the wounds became badly infected and in cases where the animals lived, the skin between the partners stretched and became twisted.(This particular disadvantage of the Sauerbruck and Heyde method is illustrated in Schmidt's paper ('22)). A study of the unsatisfactory results which we obtained using the method as described by Sauerbruck and Heyde convinced us that the infections and deaths were caused in most of the cases by the animals pulling on the sutures of the skin. We, therefore, concluded that the union between the animals must be made more substantial than can be done by skin and muscle union. Accordingly, the