Pdgfr-α mediates testis cord organization and fetal Leydig cell development in the XY gonad
Pdgfr-α mediates testis cord organization and fetal Leydig cell development in the XY gonad
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DOI：
10.1101/gad.1052503
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发表时间：
2003-03-15
影响因子：
10.5
通讯作者：
Capel, B
中科院分区：
文献类型：
--
作者：
Brennan, J;Tilmann, C;Capel, B
在睾丸发育过程中，Sry引发的快速形态变化需要多种信号通路的协调整合。基于血小板衍生生长因子(PDGF)家族配体和受体在不同器官系统的细胞迁移、增殖和分化中的作用，我们研究了PDGF在睾丸器官发生中的作用。对PDGFR-α(-/-)胚胎的表达模式和性腺表型的分析表明，PDGFR-a在睾丸发育的多个阶段是早期睾丸信号转导的关键介质。PDGFR-α(-/-)XY性腺在血管系统的组织结构以及间质和睾丸索间隔区的划分中显示出破坏。进一步检查发现，特征性的XY增殖、中肾细胞迁移和胎儿间质细胞分化严重减少。这项工作发现，通过α受体的PDGF信号是Sry下游在睾丸器官发生和间质细胞分化中的一个重要事件。
During testis development, the rapid morphological changes initiated by Sry require the coordinate integration of many signaling pathways. Based on the established role of the platelet-derived growth factor (PDGF) family of ligands and receptors in migration, proliferation, and differentiation of cells in various organ systems, we have investigated the role of PDGF in testis organogenesis. Analysis of expression patterns and characterization of the gonad phenotype in Pdgfr-alpha(-/-) embryos identified PDGFR-a as a critical mediator of signaling in the early testis at multiple steps of testis development. Pdgfr-alpha(-/-) XY gonads displayed disruptions in the organization of the vasculature and in the partitioning of interstitial and testis cord compartments. Closer examination revealed severe reductions in characteristic XY proliferation, mesonephric cell migration, and fetal Leydig cell differentiation. This work identifies PDGF signaling through the alpha receptor as an important event downstream of Sry in testis organogenesis and Leydig cell differentiation.