The effects of ectomycorrhizal fungi on heavy metals' transport in Pinus massoniana and bacteria community in rhizosphere soil in mine tailing area.
The effects of ectomycorrhizal fungi on heavy metals' transport in Pinus massoniana and bacteria community in rhizosphere soil in mine tailing area.
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DOI：
10.1016/j.jhazmat.2019.121203
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发表时间：
2020-01
影响因子：
13.6
通讯作者：
P. Yu;Youping Sun;Zhongliang Huang;F. Zhu;Yujing Sun;Lijuan Jiang
中科院分区：
文献类型：
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作者：
P. Yu;Youping Sun;Zhongliang Huang;F. Zhu;Yujing Sun;Lijuan Jiang
马尾松是尾矿废弃地造林的潜在树种之一，外生菌根真菌对其成活有显著的促进作用。然而，外生菌根真菌与马尾松在重金属胁迫下的相互作用机制尚不清楚。设计了两个系统（接种和不接种外生菌根真菌的马尾松），每个系统包括根际和非根际，以全土为对照。结果表明，接种外生菌根真菌处理能显著提高土壤水分、全碳/全氮和养分的吸收，降低土壤容重和重金属含量（p<0. 05）。有外生菌根菌的植物根系对重金属的积累量大于无外生菌根菌的植物根系。相反，它们在芽与外生菌根真菌较低。细菌群落隶属于23门70纲115目201科363属。约束主坐标分析和冗余度分析表明，接种和未接种外生菌根真菌的土壤和非接种外生菌根真菌的土壤中细菌群落结构差异明显，但根际和非根际细菌群落结构差异不大。LEfSe分析表明，酸性细菌、放线菌和变形菌是造成处理间差异的优势菌门。
Pinus massonianais one of the potential tree species of afforestation in barren mine tailing area, and ectomycorrhizal fungi contributes remarkably to its survival. However, how ectomycorrhizal fungi interacts withPinus massonianaunder heavy metals’ stress is unknown. Two systems (Pinus massonianainoculated with and without ectomycorrhizal fungi) were designed, and each system contained rhizosphere and non-rhizosphere, while bulk soil was sampled as control. The results showed that treatment of ectomycorrhizal fungi inoculation could obviously improved the absorption of soil moisture, total carbon/total nitrogen and nutrients, while reduced the bulk density and heavy metals of soil when compared with control (p<0.05). The heavy metals accumulating in plants’ roots with ectomycorrhizal fungi were greater than that without ectomycorrhizal fungi. Conversely, they were lower in shoots with ectomycorrhizal fungi. The bacterial community were affiliated with 23 bacterial phyla, 70 classes, 115 orders, 201 families, and 363 genera. Constrained Principal Coordinate Analysis and redundancy analysis demonstrated that bacterial communities structure in the soil inoculated with or without ectomycorrhizal fungi and bulk soil were distinguishable, but no difference between rhizosphere and non-rhizosphere. The LEfSe analysis showedAcidobacteria,Actinobacteria,andProteobacteriawere the dominant phyla that contributed to the difference among treatments.