TRANSFER OF YEAST TELOMERES TO LINEAR PLASMIDS BY RECOMBINATION
TRANSFER OF YEAST TELOMERES TO LINEAR PLASMIDS BY RECOMBINATION
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DOI：
10.1016/0092-8674(84)90205-8
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
SZOSTAK, JW
中科院分区：
文献类型：
--
作者：
DUNN, B;SZAUTER, P;SZOSTAK, JW
其中之一描述了线性质粒和酵母染色体端粒相邻序列（Y''元件）之间的两种类型的重组事件。在一种类型的事件中，Y”元件内的线性质粒限制性切割重新获得了缺失的 Y”DNA，并且还可能获得额外的 Y”元件。这个过程类似于通过重组修复断裂的染色体。在第二种类型的事件中，线性质粒上末端添加的 C1-3A 序列与位于染色体 Y'' 元件内部的 C1-3A 序列相互作用，导致向质粒添加 1-4 个 Y'' 元件。不同染色体末端之间发生的类似重组事件可能导致端粒相邻序列的分散和扩增。
One characterized 2 types of recombination events between linear plasmids and yeast chromosomal telomere-adjacent sequences (Y'' elements). In one type of event, a linear plasmid restriction-cut within a Y'' element regains the missing Y'' DNA, and may also acquire additional Y'' elements. This process is similar to the healing of broken chromosomes by recombination. In a second type of event, terminally added C1-3A sequences on the linear plasmid interact with C1-3A sequences located just internal to the chromosomal Y'' elements, resulting in the addition of 1-4 Y'' elements to the plasmid. Similar recombination events occurring between different chromosome ends could lead to the dispersal and amplification of telomere-adjacent sequences.