Interaction of brain cytoplasmic dynein and MAP2 with a common sequence at the C terminus of tubulin
Interaction of brain cytoplasmic dynein and MAP2 with a common sequence at the C terminus of tubulin
复制标题
脑细胞质动力蛋白和 MAP2 与微管蛋白 C 末端共同序列的相互作用
作者：
B. Paschal;R. Obar;R. Vallee
在神经元细胞中已经鉴定出两种主要类型的微管相关蛋白（MAP）。纤维状MAP，包括MAP 2和tau，用于组织和调节微管的组装。第二类不同的产生力的MAP，包括驱动蛋白1，动力蛋白2 -4和动力蛋白5，参与微管为基础的运动。这些蛋白质是机械化学ATP酶，它们似乎负责细胞器的双向运输，也可能负责染色体的运动。在这里，我们报告说，MAP 2抑制微管动力蛋白包被的盖玻片上的滑动，以及微管激活的ATP酶的动力蛋白，表明MAP 2和其他纤维MAPs可能是重要的调制器的微管为基础的运动在体内。通过微管蛋白的蛋白水解修饰，我们发现动力蛋白在α-和β-微管蛋白的C末端与微管相互作用，该区域先前被报道为MAP相互作用的位点26，7。定点抗体的使用暗示α-和β-微管蛋白的一个小区域，包含序列Glu-Gly-Glu-Glu，作为动力蛋白和MAP 2与微管相互作用的位点。
TWO main types of microtubule-associated proteins (MAPs) have been identified in neuronal cells. The fibrous MAPs, including MAP2 and tau, serve to organize and regulate the assembly of microtubules. A second distinct class of force-producing MAPs, including kinesin1, dynein2–4 and dynamin5, are involved in micro-tubule-based movement. These proteins are mechanochemical ATPases which seem to be responsible for the bidirectional transport of organelles and perhaps also the movement of chromosomes. Here we report that MAP2 inhibits microtubule gliding on dynein-coated coverslips, as well as the microtubule-activated ATPase of dynein, indicating that MAP2 and other fibrous MAPs could be important modulators of microtubule-based motility in vivo. By proteolytic modification of tubulin, we found that dynein interacts with microtubules at the C termini of α- and β-tubulin, the regions previously reported to be the sites for the interaction of MAP26,7. The use of site-directed antibodies implicates a small region of α- and β-tubulin, containing the sequence Glu-Gly-Glu-Glu, as the site of the interaction of dynein and MAP2 with the microtubule.
DOI：
10.1016/s0021-9258(18)61070-1
发表时间：
1987-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Matsudaira
通讯作者：
P. Matsudaira
无需组装促进剂即可对微管进行可逆组装纯化，并进一步纯化微管蛋白、微管相关蛋白和 MAP 片段。
影响因子：
--
作者：
Vallee,RB
通讯作者：
Vallee,RB
蛋白水解裂解的微管蛋白的组装。
影响因子：
3.9
作者：
Brown,HR;Erickson,HP
通讯作者：
Erickson,HP