Ionic-Wind-Enhanced Raman Spectroscopy without Enhancement Substrates.
Ionic-Wind-Enhanced Raman Spectroscopy without Enhancement Substrates.
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DOI：
10.1021/acs.analchem.2c04189
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发表时间：
2022-12
影响因子：
7.4
通讯作者：
Qingyou Liang;Xiangjun Gong;Jinchao Liu;C. Ke;Jie-Ming Dong;Guosheng Song;Pu Feng;Huakang Yu;Xianfeng Yang;J. Cui;Chunlin Deng;Zhiyuan Li;Shih-Cheng Liu;Guangzhao Zhang
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文献类型：
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作者：
Qingyou Liang;Xiangjun Gong;Jinchao Liu;C. Ke;Jie-Ming Dong;Guosheng Song;Pu Feng;Huakang Yu;Xianfeng Yang;J. Cui;Chunlin Deng;Zhiyuan Li;Shih-Cheng Liu;Guangzhao Zhang
拉曼光谱常被强荧光所掩盖，严重阻碍了拉曼光谱的应用。在此，我们第一次报告离子风增强拉曼光谱（IWERS）与光漂白（PB）作为一种非侵入性的方法来检测荧光和脆弱的样品没有基板。在这项研究中，离子风（IW）所产生的针-网电极转移到样品表面在空气中的表面增强拉曼光谱在毫米级，而不是纳米。密度泛函理论计算表明，离子阱中的离子粒子增加了样品分子的磁化率，从而增强了拉曼信号。同时，IW与PB的掺入产生协同效应以猝灭荧光。因此，这种方法可以将拉曼峰的信噪比提高到仅PB的三倍。同时，IWERS可以避免样品污染和破坏，无需衬底以及高激光功率。对于考古样本和类似于火星地质样本的红色岩石，IWERS成功地识别了微弱但关键的拉曼峰，这些峰被强烈的荧光所掩盖。这表明，IWERS是一个很有前途的工具，在考古学，行星科学，生物医学和软物质领域的表征。
Raman spectra are often masked by strong fluorescence, which severely hinders the applications of Raman spectroscopy. Herein, for the first time, we report ionic-wind-enhanced Raman spectroscopy (IWERS) incorporated with photobleaching (PB) as a noninvasive approach to detect fluorescent and vulnerable samples without a substrate. In this study, ionic wind (IW) generated by needle-net electrodes transfers charges to the sample surface in air on the scale of millimeters rather than nanometers in surface-enhanced Raman spectroscopy. Density functional theory calculations reveal that the ionic particles in IW increase the susceptibility of the sample molecules, thus enhancing the Raman signals. Meanwhile, the incorporation of IW with PB yields a synergistic effect to quench fluorescence. Therefore, this approach can improve the signal-to-noise ratio of Raman peaks up to three times higher than that with only PB. At the same time, IWERS can avoid sample pollution and destruction without substrates as well as high laser power. For archeological samples and a red rock as an analogue to Mars geological samples, IWERS successfully identified weak but key Raman peaks, which were masked by strong florescence. It suggests that IWERS is a promising tool for characterizations in the fields of archeology, planetary science, biomedicine, and soft matter.