Surface Immobilized Nucleic Acid-Transcription Factor Quantum Dots for Biosensing
Surface Immobilized Nucleic Acid-Transcription Factor Quantum Dots for Biosensing
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DOI：
10.1002/adhm.202000403
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发表时间：
2020-07-21
影响因子：
10
通讯作者：
Grinstaff, Mark W.
中科院分区：
文献类型：
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作者：
Chen, Mingfu;Nguyen, Thuy T.;Grinstaff, Mark W.
将生物传感器固定在表面上是开发用于实际应用的设备的关键一步。这里的制备，表征和评价的表面结合的转录因子-核酸复合物的分析物检测作为替代传统的系统采用适体或抗体进行描述。该传感器由巯基化Cy 5荧光团标记的DNA修饰的金表面和连接到量子点（QD）的变构转录因子（TetR）组成。在加入脱水四环素（aTc）-分析物-TetR-QD从表面结合的DNA释放，导致福斯特共振能量转移信号的损失。该传感器响应在0-200 μ mTc的相关范围内的剂量依赖性的方式与80 nm的检测限。传感器的制造和随后的实时定量测量建立了一个框架，用于设计未来的表面结合的，基于亲和力的生物传感器，使用变构转录因子的分子识别。
Immobilization of biosensors on surfaces is a key step toward development of devices for real-world applications. Here the preparation, characterization, and evaluation of a surface-bound transcription factor-nucleic acid complex for analyte detection as an alternative to conventional systems employing aptamers or antibodies are described. The sensor consists of a gold surface modified with thiolated Cy5 fluorophore-labeled DNA and an allosteric transcription factor (TetR) linked to a quantum dot (QD). Upon addition of anhydrotetracycline (aTc)-the analyte-the TetR-QDs release from the surface-bound DNA, resulting in loss of the Forster resonance energy transfer signal. The sensor responds in a dose-dependent manner over the relevant range of 0-200 mu maTc with a limit of detection of 80 nm. The fabrication of the sensor and the subsequent real-time quantitative measurements establish a framework for the design of future surface-bound, affinity-based biosensors using allosteric transcription factors for molecular recognition.