Rendering the Intractable More Tractable: Tools from Caenorhabditis elegans Ripe for Import into Parasitic Nematodes.
Rendering the Intractable More Tractable: Tools from Caenorhabditis elegans Ripe for Import into Parasitic Nematodes.
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DOI：
10.1534/genetics.115.182717
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发表时间：
2015-12
期刊：
影响因子：
3.3
通讯作者：
Ward JD
中科院分区：
文献类型：
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作者：
Ward JD
遗传和分子工具的最新快速进展为秀丽隐杆线虫带来了惊人的易处理性，这是一种最初因其简单的设计和易于成像而受到珍视的模型。C.线虫长期以来一直是生物医学研究中的强大模型，RNAi和CRISPR/Cas9系统等工具分别允许轻松敲除基因和编辑基因组。这些发展为解决全球健康和粮食安全最严重的负担之一：寄生线虫创造了额外的机会。我回顾如何发展的非寄生线虫的遗传模型通知努力进口工具到寄生线虫。目前在三种常用的研究寄生虫（类圆线虫属，马来丝虫（Brugia malayi）和猪蛔虫（Ascaris suum），以及C.适应环境的时机已经成熟，以及这样做的好处和障碍。这些工具将使解剖的一个巨大的阵列的问题，一直是所有，但完全无法穿透的日期，允许调查宿主寄生虫和寄生虫载体的相互作用，以及寄生的遗传基础。
Recent and rapid advances in genetic and molecular tools have brought spectacular tractability to Caenorhabditis elegans, a model that was initially prized because of its simple design and ease of imaging. C. elegans has long been a powerful model in biomedical research, and tools such as RNAi and the CRISPR/Cas9 system allow facile knockdown of genes and genome editing, respectively. These developments have created an additional opportunity to tackle one of the most debilitating burdens on global health and food security: parasitic nematodes. I review how development of nonparasitic nematodes as genetic models informs efforts to import tools into parasitic nematodes. Current tools in three commonly studied parasites (Strongyloides spp., Brugia malayi, and Ascaris suum) are described, as are tools from C. elegans that are ripe for adaptation and the benefits and barriers to doing so. These tools will enable dissection of a huge array of questions that have been all but completely impenetrable to date, allowing investigation into host–parasite and parasite–vector interactions, and the genetic basis of parasitism.