Identification and Biological Role of the Endosymbionts Wolbachia in Rice Water Weevil (Coleoptera: Curculionidae)
Identification and Biological Role of the Endosymbionts Wolbachia in Rice Water Weevil (Coleoptera: Curculionidae)
复制标题
稻水象甲内共生菌沃尔巴克氏体的鉴定及其生物学作用（鞘翅目：象甲科）
DOI：
10.1603/en11195
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发表时间：
2012-06-01
影响因子：
1.7
通讯作者：
Way, Michael O.
中科院分区：
文献类型：
--
作者：
Chen, Shu-Juan;Lu, Fang;Way, Michael O.
沃尔巴克氏菌属是存在于许多节肢动物物种的生殖组织中的专性细胞内细菌。在中国引进的稻水象甲 Lissorhoptrus oryzophilus Kuschel（鞘翅目，象甲科）中研究了沃尔巴克氏体的感染状况及其在宿主繁殖中的作用。我们检查了中国 5 个以孤雌生殖方式繁殖的种群以及德克萨斯州东南部的一个以双性繁殖方式繁殖的本地种群中沃尔巴克氏体的感染状况。所有种群均感染沃尔巴克氏体，且每个种群的所有标本均感染单一菌株的沃尔巴克氏体。基于多位点序列分型系统的系统发育分析表明，非本地稻米象鼻虫中的Wolbachia与本地象鼻虫中的Wolbachia存在明显差异。用四环素处理后，孤雌象鼻虫的繁殖力显着降低，卵不能存活。我们的结果表明，沃尔巴克氏体对于米嗜乳杆菌卵母细胞的产生是必需的。
Wolbachia spp. are obligate intracellular bacteria present in reproductive tissues of many arthropod species. Wolbachia infection status and roles in host reproduction were studied in the rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera, Curculionidae), an introduced species in China. We examined Wolbachia infection status in five populations in China where it reproduces parthenogenetically, and one native population in Southeast Texas, where it reproduces bisexually. All populations were infected by Wolbachia, and all specimens in each population were infected by Wolbachia of a single strain. Phylogenetic analyses based on multilocus sequence typing system indicated that Wolbachia in non-native L. oryzophilus weevils diverges evidently from those in native weevils. After treatments with tetracycline, parthenogenetic weevils reduced the fecundity significantly and eggs were not viable. Our results suggest that Wolbachia are necessary for oocyte production in L oryzophilus.