Induction of pulmonary allergen-specific IgA responses or airway hyperresponsiveness in the absence of allergic lung disease following sensitization with limiting doses of ovalbumin-alum
Induction of pulmonary allergen-specific IgA responses or airway hyperresponsiveness in the absence of allergic lung disease following sensitization with limiting doses of ovalbumin-alum
复制标题
DOI：
10.1006/cimm.2001.1854
复制
发表时间：
2001-09-15
影响因子：
4.3
通讯作者：
Gordon, JR
中科院分区：
文献类型：
--
作者：
Schneider, AM;Li, F;Gordon, JR
呼吸道过敏是指对无害的外来物质不能产生非致病性反应。在此，我们评估了过敏原的致敏剂量在这种反应/无反应范例中的作用，用5 ng-2 μ g的OVA-明矾致敏BALB/c小鼠，并评估它们对反复OVA气雾剂攻击的反应。用小于或等于25 ng的OVA-明矾致敏的小鼠没有产生特应性抗体、气道高反应性（AHR）、嗜酸性粒细胞增多症或肺Th 2反应，但25 ng组动物确实产生了显著的伊加反应。用100 ng OVA-明矾致敏的小鼠在没有可检测的过敏性疾病的情况下发展AHR，而用250 ng-2 μ g OVA/明矾致敏的小鼠发展全谱过敏性疾病（即，嗜酸性粒细胞增多症、IgE、IgG 1、肺Th 2细胞因子应答和AHR）。这些数据表明，有限剂量的过敏原可以差异诱导伊加或AHR在小鼠的特应性疾病的情况下。(C)2001年，爱思唯尔科学。
Respiratory allergies represent a failure to generate nonpathogenic responses to innocuous foreign materials. Herein we assessed the role of the sensitizing dose of allergen in this response/nonresponse paradigm, sensitizing BALB/c mice with 5 ng-2 mug of OVA-alum and assessing their responses to repeated OVA aerosol challenge. Mice sensitized with less than or equal to25 ng of OVA-alum did not develop atopic antibodies, airway hyper-responsiveness (AHR), eosinophilia, or pulmonary Th2 responses, but the 25-ng group animals did develop significant IgA responses. The mice sensitized with 100 ng of OVA-alum developed AHR in the absence of detectable allergic disease, while the mice sensitized with 250 ng-2 mug of OVA/alum developed full-spectrum allergic disease (i.e., eosinophilia, IgE, IgG1, pulmonary Th2 cytokine responses, and AHR). These data indicate that limiting doses of allergen can differentially induce IgA or AHR in the absence of atopic disease in mice. (C) 2001 Elsevier Science.