Nutrients Availability Shapes Fungal Community Composition and Diversity in the Rare Earth Mine Tailings of Southern Jiangxi, China
Nutrients Availability Shapes Fungal Community Composition and Diversity in the Rare Earth Mine Tailings of Southern Jiangxi, China
复制标题
养分有效性影响中国赣南稀土尾矿的真菌群落组成和多样性
DOI：
10.1134/s1067413618660037
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发表时间：
2018-11-01
影响因子：
1.1
通讯作者：
Wu, Jichun
中科院分区：
文献类型：
--
作者：
He, Genhe;Wang, Xiaodong;Wu, Jichun
以赣南3个不同时期废弃的稀土尾矿库为研究对象，采用ITS rRNA序列分析方法，研究了土壤养分的有效性及其对真菌组成和多样性的影响。对三种尾矿（RS 1、RS 2和RS3）和一种参考土壤（RS 4）进行了采样。结果表明，RS 1和RS 4的AN和TOC含量较高，RS 2和RS 3的SO 42-、TN、NO3--N、NH 4 +-N和稀土元素含量较高，表明不同尾矿库的环境异质性较高。宏基因组分析表明，RS 1 RS 4分别含有355、564、366和507个操作分类单位（OTU）。Alpha多样性分析表明，对照样品RS 4的Shannon多样性最高，而新形成的尾矿RS 3的Chao 1丰富度最低。在监测的环境变量中，AN和SO 42-是影响真菌群落总体组成和特定谱系相对丰度的最重要因素。RS 1和RS 4中以青霉菌属为主，RS 2和RS 3中以曲霉菌属为主，说明不同尾矿中硝化作用可能由不同真菌属进行。这些结果表明，稀土尾矿中营养元素的恶化，特别是硫酸盐含量的增加和有效氮含量的降低，可能是导致真菌群落组成和多样性降低以及真菌主导的异养硝化作用改变的原因。
The present study characterized the nutrients availability of three rare earth tailings deserted in different time stages in Southern Jiangxi of China, and revealed the influence of the soil nutrient properties on fungi composition and diversity by using an internal transcribed spacer (ITS) rRNA sequence approach. Three tailings (RS1, RS2 and RS3) and one reference soil (RS4) were sampled. Results showed that a higher levels of AN and TOC occurred in RS1 and RS4, whereas higher contents of SO42-, TN, NO3--N, NH4+-N and rare earth elements (REEs) existed in RS2 and RS3, suggesting a high environmental heterogeneity in the different tailings. Metagenomic analysis showed that RS1RS4 harbored 355, 564, 366 and 507 operational taxonomic units (OTUs), respectively. Alpha diversity analysis showed that RS4, a control sample, had the highest Shannon diversity, whereas RS3, a newly formed tailings, exhibited the lowest Chao1 richness.Among the monitored environmental variables, AN and SO42- were the most important factors affecting the overall fungal community composition and the relative abundances of specific lineages. Additionally, genus Penicillium prevailed in RS1and RS4 while genus Aspergillus dominated in RS2 and RS3, indicating that the nitrification maybe conduct by different fungal genus in different tailings. Together, these results suggest that lower fungal community composition and diversity as well as the alteration in fungi-dominated heterotrophic nitrification may be due to the deteriorative nutrient in rare earth tailings, especially increasing level of sulfate and decreasing level of available nitrogen.