Identification of Streptococcus mutans PAc peptide motif binding with human MHC class II molecules (DRB1*0802, *1101, *1401 and *1405)
Identification of Streptococcus mutans PAc peptide motif binding with human MHC class II molecules (DRB1*0802, *1101, *1401 and *1405)
复制标题
鉴定变形链球菌 PAc 肽基序与人 MHC II 类分子的结合（DRB1*0802、*1101、*1401 和 *1405）
作者：
Senpuku;Yanagi;Nisizawa
变形链球菌的表面蛋白抗原（PAc），特别是该PAc分子的A区，已被认为是有效龋齿疫苗研究的可能靶点。为了鉴定A区中与主要组织相容性复合体（MHC）II类（HLA-DR）分子结合的抗原肽，我们在A区的第二个单元中制备了一组重叠的合成肽，并建立了一种简单的酶联免疫吸附试验（ELISA）结合试验，可以通过孵育DR粗品来实现。通过使用ELISA结合测定研究了来自9个供体的这些肽与DR分子的结合。结果显示，在9名受试者中的7名中，PAc（316-334）肽与HLA-DR分子的结合更强。特别地，鉴定了与PAc（316-334）肽强烈结合的DR 8（DRB 1 *0802）、DR 5（DRB 1 * 1101）和DR 6（DRB 1 *1402和 *1405）。此外，我们合成了该肽的甘氨酸取代的肽类似物，并检查了结合区的结合基序。因此，在L-RV-K-A中发现了DR 8、DR 5和DR 6中的多结合基序。建议通过将多结合基序与B细胞表位组合以仅产生针对龋齿的抑制性抗体来设计对人类更有效且副作用更少的针对龋齿的肽疫苗。因此，该肽可用于一般人群中的肽疫苗开发。
A surface protein antigen (PAc) of Streptococcus mutans, in particular the A‐region of this PAc molecule, has been noted as a possible target in research for an effective dental caries vaccine. To identify the antigenic peptide binding to major histocompatibility complex (MHC) class II (HLA‐DR) molecules in the A‐region, we prepared a panel of overlapping synthetic peptides in the second unit of the A‐region, and established that a simple enzyme‐linked immunosorbent assay (ELISA) binding assay could be achieved by incubating the DR‐crude. Binding to DR molecules of these peptides from nine donors was investigated by using the ELISA binding assay. It was revealed that the PAc(316–334) peptide bound more strongly to the HLA‐DR molecule in seven out of nine subjects. In particular, DR8 (DRB1*0802), DR5 (DRB1*1101) and DR6(DRB1*1402 and*1405), which bound strongly to PAc(316–334) peptide, were identified. Moreover, we synthesized glycine‐substituted peptide analogues of the peptide and examined the binding motif of the binding region. As a result, the multiple binding motif in DR8, DR5 and DR6 was found in L‐RV‐K‐A. It is suggested that a peptide vaccine for dental caries that is more effective for humans, with fewer adverse side‐effects, could be designed by combining the multiple binding motif with the B‐cell epitope to produce only the inhibiting antibody against dental caries. The peptide could therefore be useful for peptide vaccine development in the general human population.
流感血凝素衍生肽与纯化的 HLA-DR 高亲和力结合。
DOI：
--
发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Roche,PA;Cresswell,P
通讯作者：
Cresswell,P
DOI：
10.1073/pnas.89.19.8995
发表时间：
1992
影响因子：
11.1
作者：
Ogasawara,K;Naruse,H;Itoh,Y;Gotohda,T;Arikawa,J;Kida,H;Good,RA;Onoé,K
通讯作者：
Onoé,K
链球菌表面蛋白抗原的合成肽的免疫原性和对变形链球菌口腔定植的保护作用。
DOI：
--
发表时间：
1991
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Takahashi,I;Okahashi,N;Matsushita,K;Tokuda,M;Kanamoto,T;Munekata,E;Russell,MW;Koga,T
通讯作者：
Koga,T