Isolation of mitochondria from the CNS.
Isolation of mitochondria from the CNS.
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DOI：
10.1002/0471142301.ns0722s52
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发表时间：
2010-07
影响因子：
--
通讯作者：
Kristian, Tibor
中科院分区：
文献类型：
--
作者：
Kristian, Tibor
该单元包含描述通过使用不连续Percoll梯度离心分离脑线粒体的方案。Percoll密度梯度离心将组织均质化过程中从细胞释放的突触体、髓磷脂和游离非突触线粒体分离成单独的级分。捕获在突触体中的线粒体（突触线粒体）可以使用氮气空化释放，然后通过Percoll梯度离心进一步纯化。这些方法产生表现出良好的呼吸偶联和高呼吸速率的线粒体。
This unit contains a protocol describing the isolation of brain mitochondria by using discontinuous Percoll gradient centrifugation. The Percoll density gradient centrifugation separates synaptosomes, myelin, and free non-synaptic mitochondria released from cells during tissue homogenization into individual fractions. Mitochondria entrapped in synaptosomes (synaptic mitochondria) can be liberated using nitrogen cavitation and then further purified by Percoll gradient centrifugation. These methods yield mitochondria that exhibit good respiratory coupling and high respiratory rates.