Small-solute transport across specific peritoneal tissue surfaces in the rat.
Small-solute transport across specific peritoneal tissue surfaces in the rat.
复制标题
小量溶质在大鼠特定腹膜组织表面的转运。
DOI：
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发表时间：
1996
影响因子：
13.6
通讯作者：
Michael F. Flessner
中科院分区：
文献类型：
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作者：
Michael F. Flessner
基于腹膜转运的理论分析，计算了蔗糖的组织特异性传质系数（MTC），发现 MTCliver 是其他组织 MTC 大小的 5 倍。据推测，肝脏可能是腹膜透析期间小溶质最重要的单一转运器官。为了检验这一假设，将扩散室固定在大鼠盲肠、肝脏、胃或腹壁的腹膜表面，以测量扩散室中血浆和液体之间 14C-甘露醇的体内双向传质速率。已确定，对于所有四种组织，甘露醇在任一运输方向上的运输速率相似。血浆到腔室运输的 MTC 在 1.59 x 10(-3) 和 2.36 x 10(-3) cm/min 之间变化，MTCliver = 1.87 +/- 0.24 x 10(-3) cm/min。相反方向的 MTC 范围在 1.73 x 10(-3) 和 2.68 x 10(-3) cm/min 之间，MTCliver = 2.34 +/- 0.06 x 10(-3) cm/min。因此，作者关于 MTCliver 的假设被推翻。还在一系列单独的大鼠中进行腹膜透析，其中测量了解剖的腹膜组织的面积，并确定 14C-甘露醇的传质面积系数（MTAC）为 0.364 +/- 0.068 cm3/min（腔到血浆）和 0.240 +/- 0.039 cm3/min（血浆到腔）。然后将组织特异性 MTC 乘以相应的组织面积并求和以估计 1.0 cm3/min 的 MTAC，这是测量的 MTAC 的 3 至 4 倍。结论是，特定组织对血浆-腹膜转运的重要性主要取决于暴露于透析溶液的表面积。然而，只有 25% 至 30% 的解剖器官组织区域可能与透析液接触。
On the basis of a theoretical analysis of peritoneal transport, the tissue-specific mass transfer coefficients (MTC) for sucrose were calculated, and the MTCliver was found to be five times the magnitude of other tissue MTC. It was hypothesized that the liver was potentially the most significant single transport organ for small solutes during peritoneal dialysis. To test this hypothesis, diffusion chambers were affixed to the peritoneal surface of the rat cecum, liver, stomach, or abdominal wall to measure the in vivo bidirectional mass transfer rates of 14C-mannitol between the plasma and the fluid contained in the diffusion chambers. It was determined that the rate of mannitol transport in either direction of transport was similar for all four tissues. The MTC for plasma-to-chamber transport varied between 1.59 x 10(-3) and 2.36 x 10(-3) cm/min with MTCliver = 1.87 +/- 0.24 x 10(-3) cm/min. MTC in the opposite direction ranged between 1.73 x 10(-3) and 2.68 x 10(-3) cm/min with the MTCliver = 2.34 +/- 0.06 x 10(-3) cm/min. The authors' hypothesis concerning the MTCliver was therefore disproved. Peritoneal dialysis was also carried out in a separate series of rats, in which the area of the dissected peritoneal tissues was measured and the mass transfer-area coefficient (MTAC) for 14C-mannitol was determined to be 0.364 +/- 0.068 cm3/min (cavity to plasma) and 0.240 +/- 0.039 cm3/min (plasma to cavity). The tissue-specific MTC were then multiplied by the corresponding tissue areas and summed to estimate an MTAC of 1.0 cm3/min, which is 3 to 4 times the measured MTAC. It was concluded that the importance of a particular tissue to plasma-peritoneal transport is primarily dependent on the surface area exposed to the dialysis solution. However, only 25 to 30% of the dissected-organ tissue area may be in contact with the dialysate fluid.