Optimization of L-lactic Acid Production of Rhizopus Oryzae Mutant RLC41-6 by Ion Beam Implantation at Low-Energy
Optimization of L-lactic Acid Production of Rhizopus Oryzae Mutant RLC41-6 by Ion Beam Implantation at Low-Energy
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DOI：
10.1088/1009-0630/7/5/024
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发表时间：
2005-10
影响因子：
1.7
通讯作者：
Xiuhong Zhou;Chunmei Ge;Jianming Yao;R. Pan;Zengliang Yu
中科院分区：
文献类型：
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作者：
Xiuhong Zhou;Chunmei Ge;Jianming Yao;R. Pan;Zengliang Yu
为获得高产L（+）-乳酸的工业菌株，采用氮离子束注入法对根霉RF 3608进行诱变，得到一株突变株RLC 41 -6。在最佳条件下，摇瓶发酵36 h，L（+）-乳酸产量可达133 g/L ~ 137 g/L，以葡萄糖计的转化率高达88%~ 91%，生产能力为3.75 g/L·h。与出发菌株RF 3608相比，乳酸产量提高了近115%。
In order to obtain an industrial strain with a higher L(+)-lactic acid yield, the strain Rhizopus oryzae RF3608 was mutated by means of nitrogen ion beam implantation and the mutant strain RLC41-6 was isolated. Under optimal conditions the yield of L(+)-lactic acid produced in a shake-flask reached 133 g/L-137 g/L after 36 h cultivation, indicating that the conversion rate based on glucose was as high as 88%-91% and the productivity was 3.75 g/L·h. It was almost a 115% increase in lactic acid production compared with the original strain RF3608.