In African American Type 2 Diabetic Patients, Is Vitamin D Deficiency Associated with Lower Blood Levels of Hydrogen Sulfide and Cyclic Adenosine Monophosphate, and Elevated Oxidative Stress?
In African American Type 2 Diabetic Patients, Is Vitamin D Deficiency Associated with Lower Blood Levels of Hydrogen Sulfide and Cyclic Adenosine Monophosphate, and Elevated Oxidative Stress?
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DOI：
10.1089/ars.2012.4843
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发表时间：
2013-04-01
影响因子：
6.6
通讯作者：
Levine, Steven N.
中科院分区：
文献类型：
--
作者：
Jain, Sushil K.;Manna, Prasenjit;Levine, Steven N.
与高加索人相比，非洲裔美国人（AA）心血管疾病和维生素D（VD）缺乏症的发病率更高。硫化氢（H2S）是一种重要的信号分子。本研究检验了AA 2型糖尿病患者（T2D）血液中H2S水平较低的假设。从T2D和健康对照获得空腹血液。结果显示，与相应年龄和种族匹配的健康对照组相比，AA T2D患者血浆环磷酸腺苷（cAMP）和H2S水平显著降低，但高加索T2D患者血浆cAMP和H2S水平未显著降低。AA T2D患者的血浆VD水平显著低于白人T2D患者。细胞培养研究表明，1，25（OH）（2）-VD补充显著增加了高糖处理的U937单核细胞中胱硫醚-c-裂解酶（CSE）的表达、H2S形成和cAMP分泌，但降低了活性氧。这表明，VD补充上调CSE和H2S的形成，并降低氧化应激，VD缺乏可能导致H2S信号传导功能障碍，从而导致AA中血管炎症的发生率较高。这些结果导致以下假设：VD补充可以补充H2S和cAMP的血液浓度，并降低AA T2D中的氧化应激和心血管疾病。抗氧化剂。氧化还原信号。18，1154 - 1158.
African Americans (AA) have a higher incidence of cardiovascular disease and vitamin D (VD) deficiency compared with Caucasians. Hydrogen sulfide (H2S) is an important signaling molecule. This study examined the hypothesis that blood levels of H2S are lower in AA type 2 diabetic patients (T2D). Fasting blood was obtained from T2D and healthy controls. Results showed a significant decrease in plasma levels of cyclic adenosine monophosphate (cAMP) and H2S in AA T2D but not in Caucasian T2D when compared with those of respective age-and race-matched healthy controls. Plasma VD levels were significantly lower in AA T2D compared with Caucasian T2D. Cell culture studies demonstrate that 1,25(OH)(2)-VD supplementation significantly increased expression of cystathionine-c-lyase (CSE), H2S formation, and cAMP secretion, but decreased reactive oxygen species in high glucose-treated U937 monocytes. This suggests that VD supplementation upregulates CSE and H2S formation and decreases oxidative stress, and that VD deficiency may contribute to the malfunctioning of H2S signaling and thus a higher incidence of vascular inflammation in AA. These results lead to the hypothesis that VD supplementation can replenish blood concentrations of H2S and cAMP and lower oxidative stress and cardiovascular disease in AA T2D. Antioxid. Redox Signal. 18, 1154-1158.