One-step RT-PCR for detection of Zika virus
One-step RT-PCR for detection of Zika virus
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DOI：
10.1016/j.jcv.2008.05.005
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发表时间：
2008-09-01
影响因子：
8.8
通讯作者：
Sall, Amadou Alpha
中科院分区：
文献类型：
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作者：
Faye, Oumar;Faye, Ousmane;Sall, Amadou Alpha
寨卡病毒（ZIKV）是一种在亚洲和非洲流行的新型蚊媒黄病毒。人类感染引起流感样综合征，与眶后疼痛、水肿、淋巴结病或腹泻有关。寨卡热的诊断需要病毒分离和血清学，这是耗时或交叉反应的。目的：建立一种一步法RT-PCR检测人血清中ZIKV的方法。设计了靶向包膜蛋白编码区的检测方法，并对其特异性、检测限、重复性、以及检测40年来从非洲各国和宿主收集的ZIKV分离株的能力。该方法在血清和L-15培养基中的检出限和重复性分别为7.7 pfu/反应和100%;未检出其他19种黄病毒。该测定法快速、灵敏且特异性地检测细胞培养物或血清中的ZIKV，但需要使用临床样品进行验证以用于诊断。(C)2008 Elsevier B. V.保留所有权利。
Background: Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive.Objective: To develop a one-step RT-PCR assay to detect ZIKV in human serum.Study design: An assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts.Results: The assay's detection limit and repeatability were respectively 7.7 pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected.Conclusions: The assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples. (C) 2008 Elsevier B.V. All rights reserved.