FUNCTIONAL IMPLICATIONS FOR THE MICROTUBULE-ASSOCIATED PROTEIN-TAU - LOCALIZATION IN OLIGODENDROCYTES
FUNCTIONAL IMPLICATIONS FOR THE MICROTUBULE-ASSOCIATED PROTEIN-TAU - LOCALIZATION IN OLIGODENDROCYTES
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DOI：
10.1073/pnas.92.22.10369
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发表时间：
1995-10-24
影响因子：
11.1
通讯作者：
BINDER, LI
中科院分区：
文献类型：
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作者：
LOPRESTI, P;SZUCHET, S;BINDER, LI
我们提供的证据表明，微管相关蛋白 tau 存在于少突胶质细胞 (OLG) 中，少突胶质细胞是产生髓磷脂的中枢神经系统细胞。通过表明 tau 蛋白的分布模式与髓鞘碱性蛋白相似，我们的结果表明 tau 蛋白可能参与髓鞘形成的某些方面。 Tau 蛋白已在 OLG 中原位和体外得到鉴定。在束间 OLG 中，单克隆抗体 Tau-5 揭示的 tau 定位仅限于细胞体。然而，在具有丰富的突起网络的培养羊 OLG 中，在细胞体、细胞突起以及这些突起顶端的膜扩张中检测到了 tau 蛋白。此外，在此类培养物中，tau 蛋白出现在沿着细胞过程或在细胞过程末端的膜扩张中与髓磷脂碱性蛋白相邻或一致。使用荧光原位杂交记录了 tau mRNA 的存在。 tau mRNA 的分布与 tau 蛋白的分布相似。培养的 OLG 的蛋白质印迹分析显示存在许多 tau 亚型。总之，这些结果表明 tau 是一种真正的少突胶质细胞蛋白，并为确定其在这些细胞中的功能作用铺平了道路。
We present evidence that the microtubule-associated protein tau is present in oligodendrocytes (OLGs), the central nervous system cells that make myelin. By showing that tau is distributed in a pattern similar to that of myelin basic protein, our results suggest a possible involvement of tau in some aspect of myelination. Tau protein has been identified in OLGs in situ and in vitro. In interfascicular OLGs, tau localization, revealed by monoclonal antibody Tau-5, was confined to the cell somata. However, in cultured ovine OLGs with an exuberant network of processes, tau was detected in cell somata, cellular processes, and membrane expansions at the tips of these processes. Moreover, in such cultures, tau appeared localized adjacent to or coincident with myelin basic protein in membrane expansions along and at the ends of the cellular processes. The presence of tau mRNA was documented using fluorescence in situ hybridization. The distribution of the tau mRNA was similar to that of the tau protein. Western blot analysis of cultured OLGs showed the presence of many tau isoforms. Together, these results demonstrate that tau is a genuine oligodendrocyte protein and pave the way for determining its functional role in these cells.