TEX14 is essential for intercellular bridges and fertility in male mice
TEX14 is essential for intercellular bridges and fertility in male mice
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DOI：
10.1073/pnas.0505123103
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发表时间：
2006-03-28
影响因子：
11.1
通讯作者：
Matzuk, MM
中科院分区：
文献类型：
--
作者：
Greenbaum, MP;Yan, W;Matzuk, MM
体细胞的胞质分裂以中体的形成而结束，中体被剥离形成单独的子细胞。相比之下，生殖细胞胞质分裂会产生一座永久的细胞间桥梁，通过一个大的细胞质通道将子细胞连接起来。在精子发生过程中，细胞间桥梁的作用包括生殖细胞通讯、同步化和单倍体细胞中的染色体剂量补偿。虽然最近已经确定了中体的几个基本组成部分，但脊椎动物生殖细胞细胞间桥的基本组成部分到目前为止还没有被描述。在这里，我们证明了睾丸表达基因14(TEX14)是一种定位于生殖细胞胞间桥梁的新蛋白。在没有TEX14的情况下，使用电子显微镜和其他标记无法观察到细胞间桥。Tex14(-/-)小鼠的精子发生通过二倍体精原细胞的过渡放大和早期减数分裂标志物的表达而进行，但在第一次减数分裂完成之前停止。因此，TEX14是脊椎动物生殖细胞中细胞间桥所必需的，这些研究提供了证据，证明细胞间桥对精子发生和生育是必不可少的。
Cytokinesis in somatic cells concludes with the formation of a midbody, which is abscised to form individual daughter cells. In contrast, germ cell cytokinesis results in a permanent intercellular bridge connecting the daughter cells through a large cytoplasmic channel. During spermatogenesis, proposed roles for the intercellular bridge include germ cell communication, synchronization, and chromosome dosage compensation in haploid cells. Although several essential components of the midbody have recently been identified, essential components of the vertebrate germ cell intercellular bridge have until now not been described. Herein, we show that testis-ex pressed gene 14 (TEX14) is a novel protein that localizes to germ cell intercellular bridges. In the absence of TEX14, intercellular bridges are not observed by using electron microscopy and other markers. Spermatogenesis in Tex14(-/-) mice progresses through the transit amplification of diploid spermatogonia and the expression of early meiotic markers but halts before the completion of the first meiotic division. Thus, TEX14 is required for intercellular bridges in vertebrate germ cells, and these studies provide evidence that the intercellular bridge is essential for spermatogenesis and fertility.