HAPLOTYPE-SPECIFIC DIFFERENCES IN SIGNALING BY TRANSFECTED CLASS-II MOLECULES TO A LY-1+ B-CELL CLONE
HAPLOTYPE-SPECIFIC DIFFERENCES IN SIGNALING BY TRANSFECTED CLASS-II MOLECULES TO A LY-1+ B-CELL CLONE
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DOI：
10.1073/pnas.86.15.5933
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发表时间：
1989-08-01
影响因子：
11.1
通讯作者：
FRELINGER, JA
中科院分区：
文献类型：
--
作者：
BISHOP, GA;FRELINGER, JA
CH12.LX B cells are responsive to antigen-dependent differentiate signals transmitted through their surface Ek molecules. Although CH12.LX cells express surface Ak molecules do not deliver differentiate signals to these B cells. To determine whether introduction of a new A molecule into CH12.LX cells would correct this deficiency, CH12.LX cells were transfected with the genes encoding new A.alpha. and /or A.beta. molecules. It was found that transfected cells reponded to antigen-specific siganls delivered via the E.alpha.kE.beta.k, A.alpha.bA.beta.bA.beta.b, or A.alpha.kA.beta.b molecule. However, the B cells did not respond to signals generated by the molecule A.alpha.kA.beta.k, A.beta.bA.beta.k, A.alpha.kA.beta.d, or A.alpha.kA.beta.u. Comparison of these sequences suggests that two A.beta. residues, His-47 and Trp-197, are important to the transmission of differentiatie signals to B cells.
