A unique nuclear receptor direct repeat 17 (DR17) is present within the upstream region of Schistosoma mansoni female-specific p14 gene
A unique nuclear receptor direct repeat 17 (DR17) is present within the upstream region of Schistosoma mansoni female-specific p14 gene
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DOI：
10.1016/j.bbrc.2008.04.125
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发表时间：
2008-07-11
影响因子：
3.1
通讯作者：
LoVerde, Philip T.
中科院分区：
文献类型：
--
作者：
Fantappie, Marcelo Rosado;Furtado, Daniel Rodrigues;LoVerde, Philip T.
性成熟的雌性血吸虫产生的卵是疾病发病机理的原因。蛋壳前体基因p14仅在性成熟的雌性蠕虫的卵黄细胞中表达，以响应尚未识别的雄性刺激。在此，我们报告了p14基因上游区域中新型核受体反应元件的鉴定。该元素包含典型的六聚体DNA核心基序5'-puggtca，该基序由非典型的直接重复（DR17）组成。血吸虫核受体SMRXR1和SMNR1特异性与P14-DR17元件作为异二聚体结合。 SMRXR1（而不是SMNR1）作为单体绑定到该图案。 TCA核序列中突变的引入完全消除了SMRXR1/SMNR1异二聚体的结合。这一发现支持我们的假设，即曼森p14基因的表达通过核受体信号通路调节。 （c）2008 Elsevier Inc.保留所有权利。
The eggs produced by sexually mature female Schistosma mansoni are responsible for the pathogenesis of the disease. The eggshell precursor gene p14 is expressed only in the vitelline cells of sexually mature female worms in response to a yet unidentified male stimulus. Herein, we report the identification of a novel nuclear receptor response element in the upstream region of the p14 gene. This element contains the canonical hexameric DNA core motif, 5'-PuGGTCA, composed of an atypically spaced direct repeat (DR17). Schistosome nuclear receptors SmRXR1 and SmNR1 specifically bound to the p14-DR17 element as a heterodimer. SmRXR1, but not SmNR1, bound to the motif as a monomer. Introduction of mutations in the TCA core sequence completely abolished the binding by SmRXR1/SmNR1 heterodimer. This finding supports our hypothesis that the expression of Schistosoma mansoni p14 gene is regulated through the nuclear receptor signaling pathway. (C) 2008 Elsevier Inc. All rights reserved.