ZFH4 protein is expressed in many neurons of developing rat brain
ZFH4 protein is expressed in many neurons of developing rat brain
复制标题
DOI：
10.1002/cne.20382
复制
发表时间：
2005-01-31
影响因子：
2.5
通讯作者：
Sasahara, M
中科院分区：
文献类型：
--
作者：
Nogami, S;Ishii, Y;Sasahara, M
锌指同源结构域（ZFH）转录因子含有锌指基序和同源结构域，可能调节神经和间充质细胞分化。我们已经克隆了ZFH 4基因，该基因编码与ATBF 1结构密切相关的蛋白质。为了研究ZFH 4在发育中的大鼠脑中的表达模式，我们提出了针对谷胱甘肽-S-转移酶（GST）ZFH 4融合蛋白的抗体。用该抗体的蛋白质印迹法在正常大鼠脑中鉴定出390 kDa的基因产物。在胚胎期和新生期的脑干中以及新生大鼠脑中的中脑和间脑中，该蛋白质的水平较高。另外，通过北方印迹法检测到相应的12.5kb的mRNA。一项免疫定位研究表明，脑干中的有丝分裂后神经元是ZFH 4表达的主要部位，表达水平因年龄和解剖部位而异。在神经发生早期，前体细胞中的表达是短暂和微弱的。虽然ZFH 4水平在出生后下降，但ZFH 4在成熟神经元中继续表达，包括多巴脱羧酶阳性神经元。在连合下器官的非神经元细胞中也检测到高水平的表达，但在大脑皮层和海马中的成熟神经元的前体细胞中几乎检测不到表达。空间和时间的表达模式非常相似的ATBF 1，我们检测到的神经元表达ZFH 4，ATBF 1，或两者兼而有之。我们假设ZFH 4参与神经细胞成熟或脑区域特异性分化的调节。J. Comp.神经元(C)2004 Wiley-Liss，Inc.
The zinc finger-homeodomain (ZFH) transcription factors contain a zinc finger motif and a homeodomain that might regulate neural and mesenchymal cell differentiation. We have cloned the ZFH4 gene that encodes a protein with structures closely related to ATBF1. In order to study the expression pattern of ZFH4 in the developing rat brain, we raised an antibody against a glutathione-S-transferase (GST) fusion protein of ZFH4. Western blotting with this antibody identified a gene product of 390 kDa in the normal rat brain. Levels of the protein were high in the brainstem at embryonic and neonatal periods and in the midbrain and diencephalon in neonatal rat brain. In addition, the corresponding mRNA of 12.5 kb was detected by Northern blotting. An immunolocalization study showed that postmitotic neurons in the brainstem were the major site of ZFH4 expression, and the levels of expression varied depending on age and anatomical sites. Expression was transient and weak in precursor cells at early neurogenesis. Although ZFH4 levels decreased after birth, ZFH4 continued to be expressed in the mature neurons including DOPA decarboxylase-positive neurons. High levels of expression were also detected in non-neuronal cells of the subcommissural organ, but the expression was almost undetectable throughout precursor cells to mature neurons in the cerebral cortex and hippocampus. The spatial and temporal expression patterns closely resembled those of ATBF1, and we detected neurons that expressed ZFH4, ATBF1, or both. We postulate that ZFH4 participates in the regulation of neural cell maturation or of region-specific differentiation of the brain. J. Comp. Neurol. (C) 2004 Wiley-Liss, Inc.