Quantitative trait loci affecting starvation resistance in Drosophila melanogaster
Quantitative trait loci affecting starvation resistance in Drosophila melanogaster
复制标题
DOI：
10.1534/genetics.166.4.1807
复制
发表时间：
2004-04-01
期刊：
影响因子：
3.3
通讯作者：
Mackay, TFC
中科院分区：
文献类型：
--
作者：
Harbison, ST;Yamamoto, AH;Mackay, TFC
承受食物资源短缺时期的能力是一个重要的健康特征。饥饿抗性是一个受多基因互作控制的数量性状，在自然群体中表现出相当大的遗传变异。这种遗传变异可以维持在一个事实，即强选择线索之间的资源分配生殖活动和个体生存的权衡。了解影响饥饿耐受性的基因和影响自然群体中饥饿抗性变异的基因子集将使其能够从定量遗传的角度评估这一假设。我们筛选了933个共等基因P元件插入系，以确定影响饥饿耐受性的候选基因。总共383个P元件插入诱导了饥饿抗性的高度显着且通常具有性别特异性的突变变异。我们还使用了缺陷互补定位，然后互补突变，以确定12个基因的变化，在两个野生型菌株之间的饥饿抗性。我们发现的基因与卵子发生、代谢和摄食行为有关，这表明它们可能与生殖和生存有关。然而，我们也发现了基因与细胞命运的规范和细胞增殖表型，这意味着在发展过程中，在细胞水平上的资源分配也可能影响饥饿的表型反应。
The ability to withstand periods of scarce food resources is an important fitness trait. Starvation resistance is a quantitative trait controlled by multiple interacting genes and exhibits considerable genetic variation in natural populations. This genetic variation could be maintained in the fact of strong selection clue to a trade-off in resource allocation between reproductive activity and individual survival. Knowledge of the genes affecting starvation tolerance and the subset of genes that affect variation in starvation resistance in natural populations would enable its to evaluate this hypothesis from a quantitative genetic perspective. We screened 933 co-isogenic P-element insertion lines to identify candidate genes affecting starvation tolerance. A total of 383 P-element insertions induced highly significant and often sex-specific mutational variance in starvation resistance. We also used deficiency complementation mapping followed by complementation to mutations to identify 12 genes contributing to variation in starvation resistance between two wild-type strains. The genes we identified are involved in oogenesis, metabolism, and feeding behaviors, indicating a possible link to reproduction and survival. However, we also found genes with cell fate specification and cell proliferation phenotypes, which implies that resource allocation during development and at the cellular level may also influence the phenotypic response to starvation.