The TRP Ca2+ channel Yvc1 regulates hyphal reactive oxygen species gradient for maintenance of polarized growth in Candida albicans
The TRP Ca2+ channel Yvc1 regulates hyphal reactive oxygen species gradient for maintenance of polarized growth in Candida albicans
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TRP Ca2 通道 Yvc1 调节菌丝活性氧梯度以维持白色念珠菌的极化生长
DOI：
10.1016/j.fgb.2019.103282
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发表时间：
2019-12-01
影响因子：
3
通讯作者：
Li, Mingchun
中科院分区：
文献类型：
--
作者：
Peng, Liping;Yu, Qilin;Li, Mingchun
白念珠菌是一种重要的条件致病真菌，其菌丝的极性生长是其侵入性感染宿主的关键。液泡瞬时受体电位（TRP）Ca 2+通道Yvc 1和NADPH氧化酶Fre 8调控的活性氧（ROS）梯度均参与了菌丝的发育，但Yvc 1和Fre 8在菌丝极化生长过程中的关系尚待进一步研究。在此，我们发现YVC 1的缺失导致ROS沿着芽管分散分布，而在WT细胞中，它集中在菌丝尖端。此外，Fre 8的定位被改变为YVC 1被破坏。此外，与YVC 1缺失相似，加入Ca 2+螯合剂EGTA导致野生型细胞中Fre 8-GFP的去极化，表明YVC 1维持的Ca 2+梯度在Fre 8-GFP的极化分布和随后的尖端ROS梯度破坏中起关键作用。通过构建一系列GFP标记的极化生长相关蛋白，包括Bud 6，Exo 70和Lifeact，我们发现这些蛋白与Fre 8和ROS类似，在yvc 1 Delta/Delta中具有去极化定位。因此，我们的工作提供了一个机械的解释Yvc 1控制和ROS相关的菌丝极化生长，并阐明了新的光的作用，Ca 2+信号在维持氧化还原稳态和形态发生的真菌病原体。
Candida albicans is an important opportunistic fungal pathogen, and hyphal polarized growth is critical for its invasive infection to the host. Both the vacuolar transient receptor potential (TRP) Ca2+ channel Yvc1 and the NADPH oxidase Fre8-governed reactive oxygen species (ROS) gradient are involved in hyphal development, but the relationship between Yvc1 and Fre8 during hyphal polarized growth remains to be investigated. Herein, we found that deletion of YVC1 led to dispersed distribution of ROS along the germ tube, while it was concentrated at the hyphal tip in WT cells. Moreover, Fre8 localization was altered as YVC1 was disrupted. Besides, similar to deletion of YVC1, addition of the Ca2+ chelating agent EGTA caused depolarization of Fre8-GFP in the wild-type cells, indicating the critical role of Yvc1-maintained Ca2+ gradient in polarized distribution of Fre8-GFP and consequent disruption of tip ROS gradient. By constructing a series of GFP-tagged polarized growth-related proteins, including Bud6, Exo70 and Lifeact, we found that these proteins, similar to Fre8 and ROS, had depolarized localization in yvc1 Delta/Delta. Thus, our work provides a mechanic explanation of Yvc1-governed and ROS-related hyphal polarized growth, and shed a novel light on the role of Ca2+ signaling in maintenance of redox homeostasis and morphogenesis in the fungal pathogens.