The relationship of DNA double-strand break induction to radiosensitivity in human tumour cell lines.
The relationship of DNA double-strand break induction to radiosensitivity in human tumour cell lines.
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DNA双链断裂诱导与人类肿瘤细胞系放射敏感性的关系。
DOI：
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发表时间：
1990
影响因子：
2.6
通讯作者：
J. Peacock
中科院分区：
文献类型：
--
作者：
T. McMillan;A. Cassoni;S. Edwards;A. Holmes;J. Peacock
最近的数据表明，细胞系之间放射敏感性的差异可能与dsb诱导的差异有关（拉德福1986 a）。有鉴于此，我们已经着手评估在何种程度上的差异，在人类肿瘤细胞系之间的辐射存活可以归因于差异dsb诱导。对于九个人类肿瘤细胞系的存活率通过克隆形成试验进行了测定，并与通过中性滤膜洗脱测定的冰浴温度下照射的dsb诱导进行了比较。这些细胞系的敏感性差异很大，从敏感的神经母细胞瘤（2戈伊时的存活分数，SF 2 = 0.13）到耐药的膀胱癌（SF 2 = 0.62）。Dsb的诱导作用在不同的细胞系中有所不同，因此耐药细胞通常比敏感细胞受到的损伤要小。然而，损伤诱导和细胞敏感性之间的关系并不是一个简单的，和其他可能影响敏感性的因素需要调用。这些数据表明，在人类肿瘤细胞系中，放射敏感性的差异可能至少部分是由于不同水平的损伤诱导，但某些细胞系可能由于生物学特性（如修复能力）的差异而对损伤的耐受性不同。
Recent data suggest that the differences in radiosensitivity between cell lines can be related to differences in dsb induction (Radford 1986a). In the light of this we have set out to assess the extent to which differences in radiation survival between human tumour cell lines can be attributed to differences in dsb induction. For nine human tumour lines survival was assayed by clonogenic assay and compared with dsb induction by irradiation at ice-bath temperature as measured by neutral filter elution. The lines varied widely in their sensitivity, ranging from a sensitive neuroblastoma (surviving fraction at 2 Gy, SF2 = 0.13) to a resistant bladder carcinoma (SF2 = 0.62). Dsb induction was found to vary between the cell lines, such that resistant cells generally suffered less damage than sensitive ones. However, the relationship between damage induction and cellular sensitivity was not a simple one, and other factors which may influence sensitivity need to be invoked. These data suggest that, in human tumour cell lines, differences in radiosensitivity may at least in part be due to different levels of damage induction, but that some lines may vary in their tolerance of damage due to differences in biological characteristics such as repair capacity.
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更快地修复抗辐射人类肿瘤细胞中的 DNA 双链断裂。
DOI：
10.1016/0360-3016(88)90125-3
发表时间：
1988
期刊：
International journal of radiation oncology, biology, physics
影响因子：
--
作者：
Schwartz,JL;Rotmensch,J;Giovanazzi,S;Cohen,MB;Weichselbaum,RR
通讯作者：
Weichselbaum,RR
中国仓鼠 AA8 细胞和两个放射敏感克隆中伽马射线诱导的 DNA 单链和双链断裂的诱导和重新连接。
影响因子：
3.4
作者：
vanAnkeren,SC;Murray,D;Meyn,RE
通讯作者：
Meyn,RE
在 X 射线照射的有丝分裂和 G1 期 CHO 细胞中，诱导的 DNA 双链断裂水平与细胞杀伤无关。
DOI：
10.1080/09553008814552531
发表时间：
1988
期刊：
International journal of radiation biology and related studies in physics, chemistry, and medicine
影响因子：
--
作者：
Iliakis,G;Okayasu,R
通讯作者：
Okayasu,R
在适当选择裂解条件后，使用非解旋过滤器洗脱在 CHO 细胞中获得线性 DNA 洗脱剂量响应曲线。
影响因子：
2.6
作者：
Okayasu,R;Iliakis,G
通讯作者：
Iliakis,G