Differential expression of mast cell growth factor receptor (c-kit) by peritoneal connective tissue-type mast cells and tissue culture-derived mast cells.
Differential expression of mast cell growth factor receptor (c-kit) by peritoneal connective tissue-type mast cells and tissue culture-derived mast cells.
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腹膜结缔组织型肥大细胞和组织培养来源的肥大细胞差异表达肥大细胞生长因子受体（c-kit）。
DOI：
10.1002/jlb.55.5.596
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发表时间：
1994
影响因子：
5.5
通讯作者：
Ruan,Q
中科院分区：
文献类型：
--
作者：
Chen,B;Scheding,S;Nakeff,A;Ruan,Q
小鼠腹腔是结缔组织型肥大细胞（CTMCs）的主要来源。流式细胞术检测表明，腹腔内1%~ 3%的细胞具有MGFR（或c-kit），CTMCs是腹腔内唯一表达MGFR的细胞类型，并且每种细胞都表达MGFR。超过一半的腹膜CTMCs在重组鼠白细胞介素3（rMuIL-3）、rMuIL-4和rMuMGF存在下保持增殖的潜力，并产生纯的、阿辛蓝阳性的“肥大细胞”，其积极表达c-kit转录物和MGFR。流式细胞术分析和受体检测显示，在4°培养条件下，培养来源的肥大细胞（CDMCs）上MGFR的数量是腹膜CTMCs的1/3（6 104 vs. 1.8 105 MGFR/细胞）。在37°下，在CDMC中检测到的膜MGFR的总数是在4°下检测到的膜MGFR的2至3倍，这表明在4 °下，在CDMC中近70%的总MGFR作为不能与外源配体相互作用的“隐蔽位点”存在，而在腹膜CTMC中仅为40%。因此，MGFR表达减少是与CDMC相关的表型特征之一。J.Leukoc. 55：596-602; 1994.
The peritoneal cavity of the mouse is a major source of connective tissue-type mast cells (CTMCs). Flow cytometric analysis using biotinylated recombinant murine mast cell growth factor (rMuMGF) showed that 1 to 3% of the cells in the peritoneal cavity exhibited MGF receptor (MGFR) (or c-kit).CTMCs were the only cell types expressing MGFR in the peritoneal cavity, and every one of them expressed MGFR. More than half the peritoneal CTMCs retained the potential to proliferate in the presence of recombinant murine interleukin 3 (rMuIL-3), rMuIL-4, and rMuMGF and gave rise to pure, alcian blue-positive “mast cells,” which actively expressedc-kittranscripts and MGFR. Flow cytometric analysis and receptor assay carried out at 4° showed that the number of MGFRs on culture-derived mast cells (CDMCs) was one-third that of peritoneal CTMCs (6 104vs. 1.8 105MGFR/cell). At 37°, the total number of membrane MGFRs detected in CDMC was two to three times more than that detected at 4°, indicating that nearly 70% of total MGFR in CDMCs, compared with only 40% in peritoneal CTMCs, existed as “cryptic sites” unable to interact with exogenous ligand at 4°. Thus, diminished expression of MGFR is one of the phenotypic characteristics associated with CDMCs. J.Leukoc. Biol.55: 596–602; 1994.