Enhanced detection sensitivity of pegylated CdSe/ZnS quantum dots-based prostate cancer biomarkers by surface plasmon-coupled emission
Enhanced detection sensitivity of pegylated CdSe/ZnS quantum dots-based prostate cancer biomarkers by surface plasmon-coupled emission
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DOI：
10.1016/j.bios.2011.12.043
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发表时间：
2012-03-15
影响因子：
12.6
通讯作者：
Cho, Yong-Hoon
中科院分区：
文献类型：
--
作者：
Jin, Li-Hua;Li, Song-Mei;Cho, Yong-Hoon
我们通过使用增强的表面等离子体偶联的发射测量（SPCE）来证明量子点（QDS）癌蛋白生物芯片的量子点（QD）癌蛋白生物芯片的制造和检测。将PSA固定在受SIO2保护的薄金底物上，并与PSA抗体偶联的质量QD用作荧光探针。由于QD的出色亮度和发射的高方向性以及SPCE的高光收集效率，使用基于QDS的癌症蛋白质的PSA芯片的检测极限（LOD）降至10 fg/ml（等于0.3 fm）。我们预计，基于QDS的SPCE测量系统具有低LOD供应，具有检测人体内仅浓度低浓度的各种癌症生物标志物的巨大潜力。 （c）2011 Elsevier BM。版权所有。
We demonstrate the fabrication and detection of quantum dots (QDs)-based prostate specific antigens (PSAs) cancer protein biochips by using enhanced surface plasmon-coupled emission measurements (SPCE). The PSAs are immobilized on a SiO2-protected thin gold substrate and pegylated QDs which conjugated with antibodies of PSA are used as fluorescent probes. Due to the excellent brightness of the QDs and the high directionality of emission, as well as the high light collection efficiency of SPCE, the limit of detection (LOD) is down to 10 fg/mL (equal to 0.3 fM) for the PSA chips by using QDs-based cancer protein. We expect that this QDs-based SPCE measurement system with the low LOD supplies a great potential for detecting various cancer biomarkers that are present in only low concentrations within the human body. (C) 2011 Elsevier BM. All rights reserved.