CDC42P GTPASE IS INVOLVED IN CONTROLLING POLARIZED CELL-GROWTH IN SCHIZOSACCHAROMYCES-POMBE
CDC42P GTPASE IS INVOLVED IN CONTROLLING POLARIZED CELL-GROWTH IN SCHIZOSACCHAROMYCES-POMBE
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DOI：
10.1128/mcb.14.2.1075
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发表时间：
1994-02-01
影响因子：
5.3
通讯作者：
JOHNSON, DI
中科院分区：
文献类型：
--
作者：
MILLER, PJ;JOHNSON, DI
Cdc42p 是一种高度保守的低分子量 GTP 酶，参与控制细胞形态发生。我们通过其补充酿酒酵母 cdc42-1(ts) 突变的能力，从裂殖酵母裂殖酵母中分离出了 Cdc42p 同源物。粟酒裂殖酵母 Cdc42p 与酿酒酵母 Cdc42p 预测的氨基酸序列有 85% 相同，与人类 Cdc-42p 同源物有 83% 相同。 Cdc42p 蛋白分级为可溶性和颗粒级分，表明它存在于两个细胞池中。我们破坏了 cdc42(+) 基因，并表明它对于生长至关重要。 cdc42 无效表型是小、圆形、致密细胞的停滞。此外，我们在 Cdc42p GTP 结合域中生成了三个位点特异性突变：G12V、Q61L 和 D118A，这些突变对应于酿酒酵母 CDC42 中的显性致死突变。与酿酒酵母cdc42突变相比，粟酒裂殖酵母cdc42突变体等位基因在过表达时并不致死。然而，cdc42 突变体确实表现出大的畸形细胞的异常形态表型，表明粟酒裂殖酵母 Cdc42p 参与控制极化细胞生长。
Cdc42p is a highly conserved low-molecular-weight GTPase that is involved in controlling cellular morphogenesis. We have isolated the Cdc42p homolog from the fission yeast Schizosaccharomyces pombe by its ability to complement the Saccharomyces cerevisiae cdc42-1(ts) mutation. S. pombe Cdc42p is 85% identical in predicted amino acid sequence by S. cerevisiae Cdc42p and 83% identical to the human Cdc-42p homolog. The Cdc42p protein fractionates to both soluble and particulate fractions, suggesting that it exists in two cellular pools. We have disrupted the cdc42(+) gene and shown that it is essential for growth. The cdc42 null phenotype is an arrest as small, round, dense cells. In addition, we have generated three site-specific mutations, G12V, Q61L, and D118A, in the Cdc42p GTP-binding domains that correspond to dominant-lethal mutations in S. cerevisiae CDC42. In contrast to the S. cerevisiae cdc42 mutations, the S. pombe cdc42 mutants alleles were not lethal when overexpressed. However, the cdc42 mutants did exhibit an abnormal morphological phenotype of large, misshapen cells, suggesting the S. pombe Cdc42p is involved in controlling polarized cell growth.