Participation of algal surface structures in the cell recognition process during infection of aposymbiotic Paramecium bursaria with symbiotic chlorellae.
Participation of algal surface structures in the cell recognition process during infection of aposymbiotic Paramecium bursaria with symbiotic chlorellae.
复制标题
共生小球藻感染非共生草履虫期间藻类表面结构参与细胞识别过程。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
W. Wiessner
中科院分区：
文献类型：
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作者：
W. Reisser;A. Radunz;W. Wiessner
内共生单位绿色草履虫表现出很强的伴侣专一性。脱共生草履虫bursaria形成一个稳定的共生单位，只有一个特殊的菌株小球藻。藻类适合共生形成封闭在个别的藻周液泡，而不适合的藻类被隔离到食物液泡。藻类被纤毛虫识别可能是因为其特殊的表面结构，而不是它们的生理特性。同步藻类的实验表明，自体孢子被吸收到藻周空泡中的程度高于母细胞，母细胞具有不同的表面结构，如免疫学技术所示。用纤维素酶和果胶酶处理或用特异性抗体或凝集素（伴刀豆球蛋白A或蓖麻凝集素）包被的共生藻通常不被认为是合适的，并且大多数被隔离到食物泡中，尽管它们显示出与未处理的藻相同的生理特性。这些结果表明，参与碳水化合物结构的识别位点的共生寄生虫囊草履虫的相互作用，在感染过程中与特殊的受体分子在膜上的摄食泡的纤毛虫。
The endosymbiotic unit green Paramecium shows a strong specificity of its partners. The aposymbiotic Paramecium bursaria forms a stable symbiotic unit only with a special strain of Chlorella sp. Algae suitable for symbiosis formation are enclosed in individual perialgal vacuoles whereas unsuitable algae are sequestered into food vacuoles. It is probable that algae are recognized by the ciliate because of specific surface structures rather than by their physiological properties. Experiments with synchronized algae demonstrate that autospores are taken up into perialgal vacuoles to a higher degree than mother cells, which have a different surface structure as shown by immunological techniques. Symbiotic algae treated with cellulase and pectinase or having been coated with specific antibodies or with lectins (concanavalin A or Ricinus communis agglutinin) are usually not recognized as suitable and are mostly sequestered into food vacuoles although they show the same physiological properties as untreated algae. These results indicate the participation of carbohydrate structures at the recognition sites of symbiotic chlorellae in Paramecium bursaria which interact during infection with special receptor molecules in the membrane of the ingestion vacuole of the ciliate.