A nuclear protein NsiA from Epichloe festucae interacts with a MAP kinase MpkB and regulates the expression of genes required for symbiotic infection and hyphal cell fusion
A nuclear protein NsiA from Epichloe festucae interacts with a MAP kinase MpkB and regulates the expression of genes required for symbiotic infection and hyphal cell fusion
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来自羊茅的核蛋白 NsiA 与 MAP 激酶 MpkB 相互作用，调节共生感染和菌丝细胞融合所需基因的表达
DOI：
10.1111/mmi.14568
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发表时间：
2020
影响因子：
3.6
通讯作者：
Daigo Takemoto
中科院分区：
文献类型：
--
作者：
Aiko Tanaka;Shota Kamiya;Yoshino Ozaki;Shinichi Kameoka;Yuka Kayano;Sanjay Saikia;Fumitake Akano;Aiko Uemura;Hiroki Takagi;Ryohei Terauchi;Jun-ichi Maruyama;Hamzeh Haj Hammadeh;Andre Fleissner;Barry Scott;Daigo Takemoto
内生真菌Epichloë festucaesystemically定植在冷季型草的细胞间隙，以建立互利共生。宿主植物内内生菌的菌丝生长受到严格调节，并与宿主植物的生长同步。通过对共生基因的遗传筛选，筛选出了与寄主植物具有拮抗作用的突变体FR 405。感染FR 405突变体的多年生黑麦草发育迟缓，并发生早衰和死亡。FR 405中被破坏的基因编码一种核定位蛋白，称为NsiA，代表共生感染的核蛋白。像以前分离的共生突变体thensiAmutant是缺陷的菌丝细胞融合。NsiA与C2 H2锌指转录因子Ste 12和MAP激酶MpkB相互作用。两者都是其他真菌物种中细胞融合的必要成分。InE。festucae，MpkB，而不是Ste 12，是细胞融合所必需的。细胞融合和共生所需的几个基因的表达，包括proA/adv-1，pro41/ham-6，ham 7，ham 8和ham 9在thensiA突变体中下调。然而，粗糙脉孢菌中的NsiA直系同源物不是菌丝细胞融合所必需的。这些结果表明，NsiA和Ste 12直系同源物在菌丝细胞融合中的作用在真菌物种之间是独特的。
The endophytic fungusEpichloë festucaesystemically colonizes the intercellular spaces of cool‐season grasses to establish a mutualistic symbiosis. Hyphal growth of the endophyte within the host plant is tightly regulated and synchronized with the growth of the host plant. A genetic screen to identify symbiotic genes identified mutant FR405 that had an antagonistic interaction with the host plant. Perennial ryegrass infected with the FR405 mutant were stunted and underwent premature senescence and death. The disrupted gene in FR405 encodes a nuclear‐localized protein, designated as NsiA for nuclear protein for symbiotic infection. Like previously isolated symbiotic mutants thensiAmutant is defective in hyphal cell fusion. NsiA interacts with Ste12, a C2H2 zinc‐finger transcription factor, and a MAP kinase MpkB. Both are known as essential components for cell fusion in other fungal species. InE. festucae, MpkB, but not Ste12, is essential for cell fusion. Expression of several genes required for cell fusion and symbiosis, includingproA/adv‐1,pro41/ham‐6,ham7, ham8,andham9were downregulated in thensiAmutant. However, the NsiA ortholog inNeurospora crassawas not essential for hyphal cell fusion. These results demonstrate that the roles of NsiA and Ste12 orthologs in hyphal cell fusion are distinctive between fungal species.