Multifunctional Magnetic Copper Ferrite Nanoparticles as Fenton-like Reaction and Near-Infrared Photothermal Agents for Synergetic Antibacterial Therapy
Multifunctional Magnetic Copper Ferrite Nanoparticles as Fenton-like Reaction and Near-Infrared Photothermal Agents for Synergetic Antibacterial Therapy
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DOI：
10.1021/acsami.9b10096
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发表时间：
2019-09-04
影响因子：
9.5
通讯作者：
Wang, Li
中科院分区：
文献类型：
--
作者：
Liu, Yingnan;Guo, Zhirong;Wang, Li
与单一治疗相比，细菌感染的协同治疗策略因其治疗效果增强、不良反应少而受到广泛关注。在此，我们报道了一种新的协同抗菌平台，该平台将血红蛋白功能化的铜铁氧体纳米颗粒（Hb-CFNPs）的纳米催化抗菌治疗和光热治疗（PTT）结合起来。在低浓度过氧化氢（H2O2）存在下，Hb-CFNPs具有优异的Fenton和Fenton-like反应活性，能有效催化H2O2分解生成羟基自由基（中心点OH），使细菌细胞膜的通透性和对热的敏感性增加。在近红外照射下，Hb-CFNPs产生的热疗可以诱导受损细菌的死亡。此外，由于Hb-CFNPs具有优异的磁性能，通过磁富集可将光热效率提高约20倍，有利于在极低的实验剂量（20 μ g/mL）下实现优异的杀菌效果。体外抑菌实验表明，该协同抑菌策略对革兰氏阴性大肠杆菌（E. coli, 100%）和革兰氏阳性金黄色葡萄球菌（S. aureus, 96.4%）具有广谱抗菌性能。更重要的是，体内金黄色葡萄球菌感染脓肿的治疗研究表明，Hb-CFNPs可以作为一种毒性可忽略不计的候选抗菌药物，通过催化和光热效应实现细菌感染的协同治疗。因此，本研究提出了一种新型、高效、多功能的细菌感染治疗系统，为未来协同抗菌系统的设计开辟了新的途径。
Synergistic therapeutic strategies for bacterial infection have attracted extensive attentions owing to their enhanced therapeutic effects and less adverse effects compared with monotherapy. Herein, we report a novel synergistic antibacterial platform that integrates the nanocatalytic antibacterial therapy and photothermal therapy (PTT) by hemoglobin-functionalized copper ferrite nanoparticles (Hb-CFNPs). In the presence of a low concentration of hydrogen peroxide (H2O2), the excellent Fenton and Fenton-like reaction activity of Hb-CFNPs can effectively catalyze the decomposition of H2O2 to produce hydroxyl radicals (center dot OH), rendering an increase in the permeability of the bacterial cell membrane and the sensitivity to heat. With the assistance of NIR irradiation, hyperthermia generated by Hb-CFNPs can induce the death of the damaged bacteria. Additionally, owing to the outstanding magnetic property of Hb-CFNPs, it can improve the photothermal efficiency by about 20 times via magnetic enrichment, which facilitates to realize excellent bactericidal efficacy at a very low experimental dose (20 mu g/mL). In vitro antibacterial experiment shows that this synergistic antibacterial strategy has a broad-spectrum antibacterial property against Gram-negative Escherichia coli (E. coli, 100%) and Gram-positive Staphylococcus aureus (S. aureus, 96.4%). More importantly, in vivo S. aureus-infected abscess treatment studies indicate that Hb-CFNPs can serve as an antibacterial candidate with negligible toxicity to realize synergistic treatment of bacterial infections through catalytic and photothermal effects. Accordingly, this study proposes a novel, high-efficiency, and multifunctional therapeutic system for the treatment of bacterial infection, which will open up a new avenue for the design of synergistic antibacterial systems in the future.