TiO2 nanoparticles cause cell damage independent of apoptosis and autophagy by impairing the ROS-scavenging system in Pichia pastoris
TiO2 nanoparticles cause cell damage independent of apoptosis and autophagy by impairing the ROS-scavenging system in Pichia pastoris
复制标题
TiO2 纳米颗粒通过损害毕赤酵母中的 ROS 清除系统，导致独立于细胞凋亡和自噬的细胞损伤
DOI：
10.1016/j.cbi.2016.03.029
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发表时间：
2016-05-25
影响因子：
5.1
通讯作者：
Li, Mingchun
中科院分区：
文献类型：
--
作者：
Liu, Zhe;Zhang, Meng;Li, Mingchun
二氧化钛纳米颗粒的广泛应用增加了它们暴露在生态系统中的可能性，因此需要更好地了解它们对生物体的影响。然而，它们对真核生物的潜在毒性，特别是对真菌的潜在毒性，需要进一步详细的研究。在这里，我们研究了锐钛矿型二氧化钛纳米颗粒对模式真菌毕赤酵母中的ROS清除系统的影响。结果表明，纳米粒子进入细胞后对该真菌具有毒性，其毒性机制主要是细胞壁损伤、细胞膜损伤和ROS积聚，而不是细胞凋亡或自噬。有趣的是，合成的二氧化钛纳米颗粒对ROS清除系统有损害，但没有激活，这是导致细胞毒性的原因之一。此外，这种损伤与抗氧化剂相关基因的下调有关，特别是那些参与GSH调节的基因。因此，GSH可能在纳米二氧化钛与酵母细胞的相互作用中起关键作用。(C)2016爱思唯尔爱尔兰有限公司。保留所有权利。
The wide applications of titanium dioxide nanoparticles (TiO2 NPs) increase the possibility of their exposure to ecosystems, and therefore an improved understanding of their effects to organisms is required. However, their potential toxicity on eukaryotes, especially fungi, needs further detailed investigation. Here, we investigated the effects of anatase TiO2 NPs on the reactive oxygen species (ROS)-scavenging system in the model fungal organism, Pichia pastoris. Results showed that the NPs entered cells and had toxicity to this fungus, and their toxicity was attributed to cell wall damage, cell membrane damage, and ROS accumulation, but not apoptosis or autophagy. Interestingly, the synthesized TiO2 NPs impaired but not activated the ROS-scavenging system, which contributes to the cytotoxicity. Moreover, this impairment was associated with down-regulation of antioxidant-related genes, especially those genes involved in GSH regulation. Hence, GSH may play a key role in the interaction between TiO2 NPs and yeast cells. (C) 2016 Elsevier Ireland Ltd. All rights reserved.