Homodimeric monomethine cyanine dyes as fluorescent probes of biopolymers.
Homodimeric monomethine cyanine dyes as fluorescent probes of biopolymers.
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DOI：
10.1016/s1011-1344(00)00116-0
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发表时间：
2000-11
期刊：
影响因子：
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通讯作者：
I. Timtcheva;Vera Maximova;T. Deligeorgiev;N. Gadjev;K. Drexhage;I. Petkova
中科院分区：
文献类型：
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作者：
I. Timtcheva;Vera Maximova;T. Deligeorgiev;N. Gadjev;K. Drexhage;I. Petkova
研究了新合成的同二聚单次甲基花青染料在生物聚合物存在下的荧光特性。它们在 TE 缓冲液和双蒸馏水中不发出荧光，但当与 dsDNA 和 ssDNA 结合时，会在 530-650 nm 区域发出强荧光 (QF=0.3-0.9)。双链 DNA 的检测限约为 1.7 ng/ml。研究的一些染料能够在溶液和凝胶电泳中区分 dsDNA 和 ssDNA、RNA、BSA。研究了不同因素（介质的温度、pH 值和粘度、组蛋白的存在）对染料-生物聚合物复合物形成的影响。关于染料和生物聚合物之间不同类型结合的稳态和动态荧光测量结果表明，新染料可作为高灵敏度荧光标记应用于分子生物学。
The fluorescence properties of newly synthesized homodimeric monomethine cyanine dyes in the presence of biopolymers are investigated. They do not fluoresce in TE buffer and bidistilled water but become strongly fluorescent (QF=0.3–0.9) in the region 530–650 nm when bound to dsDNA and ssDNA. The detection limit of dsDNA is about 1.7 ng/ml. Some of dyes studied are able to distinguish between dsDNA and ssDNA, RNA, BSA in solution and gel electrophoresis. The influence of different factors (temperature, pH and viscosity of the medium, presence of histone) on the formation of the dye–biopolymer complexes is investigated. The results of steady-state and dynamic fluorescence measurements concerning the different types of binding between dyes and biopolymers show that the new dyes are applicable in molecular biology as highly sensitive fluorescence labels.