Detection of variant Creutzfeld-Jakob disease infectivity in extraneural tissues
Detection of variant Creutzfeld-Jakob disease infectivity in extraneural tissues
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DOI：
10.1016/s0140-6736(01)05411-3
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发表时间：
2001-07-21
期刊：
影响因子：
168.9
通讯作者：
Ironside, JW
中科院分区：
文献类型：
--
作者：
Bruce, ME;McConnell, I;Ironside, JW
朊病毒蛋白（PrP）的异常积累可以在变异型克雅氏病（vCJD）患者的脾脏、淋巴结和扁桃体中检测到，因此，已经假设，但未显示，这些组织具有感染性，这反过来又呈现了通过手术进行医源性传播的可能性。在这里，我们显示并测量了两名vCJD患者的脾脏和扁桃体中的感染性水平，通过在脑内接种的RIII小鼠中进行生物测定，类似的生物测定未能检测到血沉棕黄层和血浆中的感染性。
Abnormal accumulations of prion protein (PrP) can be detected in the spleen, lymph nodes, and tonsils of patients with variant Creutzfeldt-Jakob disease (vCJD), Therefore, it has been assumed, but not shown, that these tissues harbour infectivity, which in turn presents the potential for iatrogenic spread through surgery, Here, we show and measure levels of infectivity in spleen and tonsil from two patients with vCJD, by bioassay in intracerebrally inoculated RIII mice, Similar bioassays failed to detect infectivity in buffy coat and plasma.