Optogenetic Inhibition of Striatal GABAergic Neuronal Activity Improves Outcomes After Ischemic Brain Injury
Optogenetic Inhibition of Striatal GABAergic Neuronal Activity Improves Outcomes After Ischemic Brain Injury
复制标题
纹状体 GABA 能神经元活动的光遗传学抑制可改善缺血性脑损伤后的预后
DOI：
10.1161/strokeaha.117.019017
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发表时间：
2017-12-01
期刊：
影响因子：
8.3
通讯作者：
Wang, Yongting
中科院分区：
文献类型：
--
作者：
Jiang, Lu;Li, Wanlu;Wang, Yongting
背景和目的：纹状体GABA能神经元是成体神经发生的关键调节因子。然而，纹状体GABA能神经元活性在促进缺血性卒中后神经功能恢复中的具体作用仍不清楚。在这里，我们使用光遗传学的方法来调查这些影响和mechanism.Methods-Laser刺激通过植入光纤传递到抑制或激活Gad 2-Arch-GFP或Gad 2-ChR 2-tdTomato小鼠（n=80）纹状体GABA能神经元60分钟短暂大脑中动脉闭塞后1周。采用免疫组织化学方法检测神经功能严重程度评分、脑萎缩体积、微血管密度和细胞形态学变化。结果：与对照组相比，抑制纹状体GABA能神经元活性可改善功能恢复，减少脑萎缩体积，并抑制细胞死亡（P
Background and Purpose-Striatal GABAergic neuron is known as a key regulator in adult neurogenesis. However, the specific role of striatal GABAergic neuronal activity in the promotion of neurological recovery after ischemic stroke remains unknown. Here, we used optogenetic approach to investigate these effects and mechanism.Methods-Laser stimulation was delivered via an implanted optical fiber to inhibit or activate the striatal GABAergic neurons in Gad2-Arch-GFP or Gad2-ChR2-tdTomato mice (n=80) 1 week after 60-minute transient middle cerebral artery occlusion. Neurological severity score, brain atrophy volume, microvessel density, and cell morphological changes were examined using immunohistochemistry. Gene expression and protein levels of related growth factors were further examined using real-time polymerase chain reaction and Western blotting.Results-Inhibiting striatal GABAergic neuronal activity improved functional recovery, reduced brain atrophy volume, and prohibited cell death compared with the control (P