Sex-Specific Differences in Extracellular Vesicle Protein Cargo in Synovial Fluid of Patients with Osteoarthritis.
Sex-Specific Differences in Extracellular Vesicle Protein Cargo in Synovial Fluid of Patients with Osteoarthritis.
复制标题
骨关节炎患者滑液细胞外囊泡蛋白载重的性别差异。
DOI：
10.3390/life10120337
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发表时间：
2020-12-10
期刊：
影响因子：
--
通讯作者：
Fulzele S
中科院分区：
文献类型：
--
作者：
Kolhe R;Owens V;Sharma A;Lee TJ;Zhi W;Ghilzai U;Mondal AK;Liu Y;Isales CM;Hamrick MW;Hunter M;Fulzele S
与男性相比，女性患骨关节炎（OA）的风险明显更高。女性骨关节炎（OA）的发病机制知之甚少。细胞外囊泡（EV）已被证明在许多信号转导过程中发挥重要作用，在发病过程中的年龄相关性疾病通过旁分泌信号。对女性滑液来源的EV货物进行分子分析可能有助于发现用于治疗女性OA的新型生物标志物和治疗方法。以前，我们报道了滑液来源的EV miRNA货物在性别特异性的方式不同。本研究旨在描述OA患者滑液来源的EV蛋白负荷。我们的数据显示OA中的性别特异性EVs蛋白含量。我们发现触珠蛋白，orosomucoid，铜蓝蛋白显着上调，而载脂蛋白下调女性OA EV。在男性OA EV中，我们发现β-2-糖蛋白和补体成分5蛋白显著上调，Spt-Ada-Gcn 5乙酰转移酶（佐贺）相关因子29下调。注释、可视化和集成发现数据库（大卫）和QuickGO分析揭示了OA特异性蛋白参与几种生物、分子和细胞途径，特别是炎症过程。总之，关节滑液EV蛋白质含量的改变，在性别特异性的方式与OA，解释了增加的患病率和严重程度的OA在妇女。
Women are at a significantly higher risk of developing osteoarthritis (OA) compared to males. The pathogenesis of osteoarthritis (OA) in women is poorly understood. Extracellular vesicles (EVs) have been shown to play an essential role in numerous signaling processes during the pathogenesis of age-related diseases via paracrine signaling. Molecular profiling of the synovial fluid-derived EVs cargo in women may help in the discovery of novel biomarkers and therapeutics for the treatment of OA in women. Previously, we reported that synovial fluid-derived EV miRNA cargo differs in a sex-specific manner. This study aims to characterize synovial fluid-derived EV protein cargo in OA patients. Our data showed sex-specific EVs protein content in OA. We found haptoglobin, orosomucoid, and ceruloplasmin significantly up-regulated, whereas apolipoprotein down-regulated in female OA EVs. In males, we discovered β-2-glycoprotein, and complement component 5 proteins significantly up-regulated and Spt-Ada-Gcn5 acetyltransferase (SAGA)-associated factor 29 down-regulated in male OA EVs. Database for Annotation, Visualization, and Integrated Discovery (DAVID) and QuickGO analysis revealed OA-specific protein involvement in several biological, molecular, and cellular pathways, specifically in inflammatory processes. In conclusion, synovial fluid EV protein content is altered in a sex-specific manner with OA, explaining the increased prevalence and severity of OA in women.
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作者：
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作者：
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