Multiple replication origins with diverse control mechanisms in Haloarcula hispanica.
Multiple replication origins with diverse control mechanisms in Haloarcula hispanica.
复制标题
Haloarcula hispanica 中具有不同控制机制的多个复制起点
DOI：
10.1093/nar/gkt1214
复制
发表时间：
2014-02
影响因子：
14.9
通讯作者：
Xiang H
中科院分区：
文献类型：
--
作者：
Wu Z;Liu J;Yang H;Liu H;Xiang H
古细菌中多个复制起点的使用还不太清楚。特别是，很少有人知道他们的具体控制机制。在这里，我们调查了积极的复制起点在三个复制子的嗜盐古菌，Haloarcula hispanica，广泛的基因缺失，DNA突变和全基因组标记频率分析。我们发现，个别的起源是专门依赖于他们的共同定位的cdc 6基因，和一个单一的积极的起源/cdc 6配对是必不可少的，足以为每个复制子。值得注意的是，我们证明了oriC 1和oriC 2的活动，主染色体上的两个起源，是不同的控制。位于两个反向起点识别框（ORB）之间的内部区域中的富含G的反向重复序列充当oriC 1的增强子，而oriC 2处的复制起始由位于oriC 2-cdc 6 E下游的富含ORB的区域负调控，可能通过Cdc 6 E滴定。置于质粒上的oriC 2与野生型（而不是Δ oriC 2）宿主菌株不相容，进一步表明严格控制oriC 2活性对细胞很重要。这是第一份揭示盐古菌中不同复制起点控制机制的报告，为盐古菌中多个复制起点的使用和协调提供了新的见解。
The use of multiple replication origins in archaea is not well understood. In particular, little is known about their specific control mechanisms. Here, we investigated the active replication origins in the three replicons of a halophilic archaeon, Haloarcula hispanica, by extensive gene deletion, DNA mutation and genome-wide marker frequency analyses. We revealed that individual origins are specifically dependent on their co-located cdc6 genes, and a single active origin/cdc6 pairing is essential and sufficient for each replicon. Notably, we demonstrated that the activities of oriC1 and oriC2, the two origins on the main chromosome, are differently controlled. A G-rich inverted repeat located in the internal region between the two inverted origin recognition boxes (ORBs) plays as an enhancer for oriC1, whereas the replication initiation at oriC2 is negatively regulated by an ORB-rich region located downstream of oriC2-cdc6E, likely via Cdc6E-titrating. The oriC2 placed on a plasmid is incompatible with the wild-type (but not the ΔoriC2) host strain, further indicating that strict control of the oriC2 activity is important for the cell. This is the first report revealing diverse control mechanisms of origins in haloarchaea, which has provided novel insights into the use and coordination of multiple replication origins in the domain of Archaea.
登录
查看更多内容
西班牙盐弧菌的完整基因组序列，用于研究遗传学、代谢和病毒-宿主相互作用的盐古菌模型
影响因子：
3.2
作者：
Liu, Hailong;Wu, Zhenfang;Xiang, Hua
通讯作者：
Xiang, Hua
影响因子：
3.2
作者：
Berquist, BR;DasSarma, S
通讯作者：
DasSarma, S
嗜盐古菌中受调控的多倍体。
影响因子：
3.7
作者：
Breuert S;Allers T;Spohn G;Soppa J
通讯作者：
Soppa J
影响因子：
64.5
作者：
BRAUN, RE;ODAY, K;WRIGHT, A
通讯作者：
WRIGHT, A
DOI：
10.1073/pnas.0806414105
发表时间：
2008-10-28
影响因子：
11.1
作者：
Duggin, Iain G.;McCallum, Simon A.;Bell, Stephen D.
通讯作者：
Bell, Stephen D.