Evaluation of Cariogenic Bacteria
Evaluation of Cariogenic Bacteria
复制标题
致龋菌的评价
DOI：
10.1055/s-0039-1698309
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发表时间：
2007
影响因子：
--
通讯作者：
N. Hanada
中科院分区：
文献类型：
--
作者：
F. Nishikawara;Y. Nomura;S. Imai;A. Senda;N. Hanada
目的对变形链球菌（MS）进行评估是龋病风险的指标之一。denttoculttm和CRTTM是一种用于检测和评估MS的商用试剂盒，方便。然而，MS的评估也已经简单地使用说明书进行了。本研究的目的是比较改良的Mitis-Salivalius Bacitracin （MSB）琼脂培养基与MSB琼脂培养基和市售试剂盒的实用性，并建立一种使用改良的MSB琼脂的简易试剂盒（mMSB-kit）。方法采用MSB、改良的MSB琼脂培养基和商用试剂盒检测27例受试者刺激唾液中的质谱。实验室和临床分离的MS菌株同样被评估。采用酶联免疫吸附法比较被检细菌中质谱的含量。结果以改良的MSB琼脂培养基为基础，采用mMSB-kit进行评分。唾液样品在培养方法和商业试剂盒之间显示出不同水平的质谱。部分样品中充满质谱，商用试剂盒无法检测到。改良MSB琼脂培养基和mMSB-kit对MS的检出率显著高于MSB琼脂培养基、CRTTM （P< 0.01）和Dentocult SMTM （P< 0.05）。结论改性MSB琼脂对MS的检测灵敏度高于MSB琼脂。mmsb试剂盒使用简单，可作为评估多发性硬化症作为龋齿风险因素的重要因素。
Objectives The evaluation of Mutans streptococci (MS) is one of the index for caries risk. DentocultTM and CRTTM are commercial kits to detect and evaluate MS, conveniently. However, the evaluation of MS has also been carried out simply using an instruction manual. But the instruction manual is not easy to use for evaluation of MS. The aim of this study was to examine the utility of modified Mitis-Salivalius Bacitracin (MSB) agar medium compared with MSB agar medium and commercial kits, and to establish a convenient kit (mMSB-kit) using modified MSB agar. Methods The MS in stimulated saliva from 27 subjects were detected by MSB, modified MSB agar medium and commercial kits. Laboratory and clinically isolated strains of MS were similarly evaluated. The ratios of MS in detected bacteria were compared by ELISA. Results The scores using an mMSB-kit on the basis of modified MSB agar medium were tabulated. Saliva samples showed different levels of MS between culture methods and the commercial kit. Some samples which were full of MS were not detected by the commercial kit. The detection of MS by modified MSB agar medium and mMSB-kit were significantly higher when compared with MSB agar medium,CRTTM, (P< .01) and Dentocult SMTM (P<.05). Conclusion The sensitivity for detection of MS is higher for modified MSB agar medium when compared with MSB agar medium. The mMSB-kit can be used simply, and can be an important contributor for the evaluation of MS as a caries risk factor.
DOI：
10.17796/jcpd.26.2.t6601j3618675326
发表时间：
2002
期刊：
The Journal of clinical pediatric dentistry
影响因子：
--
作者：
F. Ramos-Gomez;J. Weintraub;S. Gansky;C. Hoover;J. Featherstone
通讯作者：
F. Ramos-Gomez;J. Weintraub;S. Gansky;C. Hoover;J. Featherstone